A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

2.50
Hdl Handle:
http://hdl.handle.net/10547/623165
Title:
A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles
Authors:
Zariwala, Mohammed Gulrez; Elsaid, Naba; Jackson, Timothy L.; Corral López, Francisco; Farnaud, Sébastien ( 0000-0003-2077-5797 ) ; Somavarapu, Satyanarayana; Renshaw, Derek ( 0000-0002-2759-0229 )
Abstract:
Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery.
Affiliation:
University of Westminster; University College London; King's College Hospital; University of Bedfordshire
Citation:
Zariwala MG, Elsaid N, Jackson TL, Corral López F, Farnaud S, Somavarapu S, Renshaw D (2013) 'A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles', International Journal of Pharmaceutics, 456 (2), pp.400-7.
Publisher:
Elsevier
Journal:
International Journal of Pharmaceutics
Issue Date:
18-Nov-2013
URI:
http://hdl.handle.net/10547/623165
DOI:
10.1016/j.ijpharm.2013.08.070
PubMed ID:
24012860
Additional Links:
https://www.sciencedirect.com/science/article/pii/S037851731300803X
Type:
Article
Language:
en
ISSN:
0378-5173
EISSN:
0378-5173
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorZariwala, Mohammed Gulrezen
dc.contributor.authorElsaid, Nabaen
dc.contributor.authorJackson, Timothy L.en
dc.contributor.authorCorral López, Franciscoen
dc.contributor.authorFarnaud, Sébastienen
dc.contributor.authorSomavarapu, Satyanarayanaen
dc.contributor.authorRenshaw, Dereken
dc.date.accessioned2019-02-15T13:45:16Z-
dc.date.available2019-02-15T13:45:16Z-
dc.date.issued2013-11-18-
dc.identifier.citationZariwala MG, Elsaid N, Jackson TL, Corral López F, Farnaud S, Somavarapu S, Renshaw D (2013) 'A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles', International Journal of Pharmaceutics, 456 (2), pp.400-7.en
dc.identifier.issn0378-5173-
dc.identifier.pmid24012860-
dc.identifier.doi10.1016/j.ijpharm.2013.08.070-
dc.identifier.urihttp://hdl.handle.net/10547/623165-
dc.description.abstractIron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S037851731300803Xen
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.subjectironen
dc.titleA novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticlesen
dc.typeArticleen
dc.identifier.eissn0378-5173-
dc.contributor.departmentUniversity of Westminsteren
dc.contributor.departmentUniversity College Londonen
dc.contributor.departmentKing's College Hospitalen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalInternational Journal of Pharmaceuticsen
dc.date.updated2019-02-15T13:42:41Z-
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