Probing action potentials of single beating cardiomyocytes using atomic force microscopy
Name:
d4ay00929k.pdf
Embargo:
2025-07-09
Size:
1.735Mb
Format:
PDF
Description:
author's accepted version
Authors
Dong, JianjunWang, Bowei
Wang, Guoliang
Zhang, Siwei
Wang, Xingyue
Wang, Rui
Crabbe, M. James C.
Wang, Zuobin
Affiliation
Changchun University of Science and TechnologyOxford University
University of Bedfordshire
Issue Date
2024-07-09Subjects
nano measurementnano-manipulation
cardiac risk-factors
Subject Categories::C560 Biotechnology
Metadata
Show full item recordAbstract
This paper presents a method for using atomic force microscopy to probe action potentials of single beating cardiomyocytes at the nanoscale. In this work, the conductive tip of an atomic force microscope (AFM) was used as a nanoelectrode to record the action potentials of self-beating cardiomyocytes in both the non-constant force contact mode and the constant force contact mode. An electrical model of a tip–cell interface was developed and the indentation force effect on the seal of an AFM conductive tip–cell membrane was theoretically analyzed. The force feedback of AFM allowed for the precise control of tip–cell contact, and enabled reliable measurements. The feasibility of simultaneously recording the action potentials and force information during the contraction of the same beating cardiomyocyte was studied. Furthermore, the AFM tip electrode was used to probe the differences of action potentials using different drugs. This method provides a way at the nanoscale for electrophysiological studies on single beating cardiomyocytes, neurons, and ion channels embedded within the cell membrane in relation to disease states, pharmaceutical drug testing and screening.Citation
Dong J, Wang B, Wang G, Zhang S, Wang X, Wang R, Crabbe MJC, Wang Z (2024) 'Probing action potentials of single beating cardiomyocytes using atomic force microscopy', Analytical Methods, 16 (32), pp.5527-5535.Publisher
Royal Society of ChemistryJournal
Analytical MethodsPubMed ID
39069789Additional Links
https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00929kType
ArticleLanguage
enISSN
1759-9660Sponsors
This work was supported by National Natural Science Foundation, Program of China (No. 62175020), National Key R&D, Program of China (No. 2023YFE0108800), Horizon Europe, Program (ENSIGN No. 101086226), Zhongshan Science and Technology Program (No. 220427204368325), Jilin Provincial Science and Technology Program (No. 20220201098GX, and 20210101038JC), and “111” Project of China (D17017).ae974a485f413a2113503eed53cd6c53
10.1039/d4ay00929k
Scopus Count
Collections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Yellow - can archive pre-print (ie pre-refereeing)