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dc.contributor.authorLi, Xuefen
dc.contributor.authorLiu, Hui
dc.contributor.authorWang, Yuxia
dc.contributor.authorCrabbe, M. James C.
dc.contributor.authorWang, Lan
dc.contributor.authorMa, Wenli
dc.contributor.authorRen, Zhumei
dc.date.accessioned2024-02-09T11:59:27Z
dc.date.available2025-02-05T00:00:00Z
dc.date.available2024-02-09T11:59:27Z
dc.date.issued2024-02-05
dc.identifier.citationLi X, Liu H, Wang Y, Crabbe MJC, Wang L, Ma W, Ren Z (2024) 'Preparation of a novel metallothionein-AuNP composite material by genetic modification and Au–S covalent combination.', International Journal of Biological Macromolecules, 262 (129960)en_US
dc.identifier.issn0141-8130
dc.identifier.pmid38325687
dc.identifier.doi10.1016/j.ijbiomac.2024.129960
dc.identifier.urihttp://hdl.handle.net/10547/626173
dc.description.abstractMetallothionein (MTs) can be used in the prevention and treatment of tumors and diabetes due to its antioxidant properties. However, it is necessary to solve its non-transmembrane properties and further improve its antioxidant activity, increase its fluorescence visualization and enhance its stability to meet practical applications in the biomedical field. Here, we report the preparation of a novel metallothionein-AuNP composite material with high transmembrane ability, fluorescence visualization, antioxidant activity, and stability by genetic modification (introducing transduction peptide TAT, fluorescence tag GFP and increasing sulfydryl groups) and immobilization technology (covalently bonding with AuNPs). The transmembrane activity of modified proteins was verified by immunofluorescence. Increasing the sulfhydryl content within a certain range can enhance the antioxidant activity of the protein. In addition, GFP were used to further simplify the imaging of the metallothionein-AuNP composite in cells. XPS results indicated that AuNPs can immobilize metallothionein through Au–S covalent bonds. TGA characterization and degradation experiments showed that thermal and degradation stability of the immobilized material was significantly improved. This work provides new ideas to construct metallothionein composites with high transmembrane ability, antioxidant activity, fluorescence visualization and stability to meet novel applications in the biomedical field.en_US
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (31672293) and Natural Science Foundation of Shanxi Province (4049920).en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.urlhttps://www.sciencedirect.com/science/article/abs/pii/S0141813024007633en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectanti-tumour effecten_US
dc.subjectenvironmental technology portfolioen_US
dc.subjectgeneticsen_US
dc.subjectSubject Categories::C400 Geneticsen_US
dc.titlePreparation of a novel metallothionein-AuNP composite material by genetic modification and Au–S covalent combinationen_US
dc.typeArticleen_US
dc.contributor.departmentShanxi Universityen_US
dc.contributor.departmentUniversity of Oxforden_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalInternational Journal of Biological Macromoleculesen_US
dc.date.updated2024-02-09T11:51:31Z
dc.description.notehttps://v2.sherpa.ac.uk/id/publication/12553 12m embargo


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