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dc.contributor.authorPang, Wei
dc.contributor.authorLu, Shuqing
dc.contributor.authorZheng, Rong
dc.contributor.authorLi, Xin
dc.contributor.authorYang, Shunbo
dc.contributor.authorFeng, Yuxia
dc.contributor.authorWang, Shuqiu
dc.contributor.authorGuo, Jin
dc.contributor.authorZhou, Shaobo
dc.identifier.citationPang W, Lu S, Zheng R, Li X, Yang S, Feng Y, Wang S, Guo J, Zhou S (2022) 'Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole', BioMed Research International, 22022 (5940372)en_US
dc.description.abstractGanoderic acid A (GAA) exhibited neuron protection in in vitro epilepsy study, but no study has been done in vivo. Rats were administered (i.p.) pentylenetetrazole daily for 28 days to induce seizure. Rats with grade II or above of epileptic score were divided into three groups and given placebo, sodium valproate, or GAA treatment, respectively, for 7 days. The electrical signals of brain were monitored with electroencephalography (EGG); epileptic behavior was assessed using the Racine scale; morphological changes and apoptosis rate of cortical neurons were assessed with H&E staining and TUNEL staining, respectively. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, and p-p38 in hippocampal tissue and Bcl-2, cleaved caspase-3, and Bax in cortical tissues was observed by Western blot and immunohistochemistry assay, respectively. After GAA treatment, apparent seizure-like EEG with significant arrhythmic disorder and spike waves was reduced or disappeared, and wave amplitude of EEG was reduced significantly. GAA showed similar effect with sodium valproate treatments on epilepsy. There were an apparent improvement of the epileptic behavior and a significant increase in the epileptic latency and shortening of the epileptic duration in the treatment group compared to control. GAA treatment ameliorated the nuclear pyknosis of neurons which appeared seriously in the epilepsy group. GAA treatment significantly reduced the cortical neuron apoptosis of epilepsy and the expression of calcium-sensing receptor, p-P38, p-JNK, cleaved caspase-3, and Bax but increased the expression of both p-ERK and Bcl-2. In conclusion, GAA treatment showed strong antiepileptic effect by decreasing apoptosis in cortical neuron and the expression of calcium-sensing receptor and stimulating the MAPK pathway.en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectGanoderic acid Aen_US
dc.subjectantiepileptic drugsen_US
dc.subjectSubject Categories::C110 Applied Biologyen_US
dc.titleInvestigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazoleen_US
dc.contributor.departmentJiamusi Universityen_US
dc.contributor.departmentQilu Hospitalen_US
dc.contributor.departmentUniversity of Greenwichen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalBioMed Research Internationalen_US
dc.description.notegold oa

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