Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole
Authors
Pang, WeiLu, Shuqing
Zheng, Rong
Li, Xin
Yang, Shunbo
Feng, Yuxia
Wang, Shuqiu
Guo, Jin
Zhou, Shaobo
Issue Date
2022-09-01
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Ganoderic acid A (GAA) exhibited neuron protection in in vitro epilepsy study, but no study has been done in vivo. Rats were administered (i.p.) pentylenetetrazole daily for 28 days to induce seizure. Rats with grade II or above of epileptic score were divided into three groups and given placebo, sodium valproate, or GAA treatment, respectively, for 7 days. The electrical signals of brain were monitored with electroencephalography (EGG); epileptic behavior was assessed using the Racine scale; morphological changes and apoptosis rate of cortical neurons were assessed with H&E staining and TUNEL staining, respectively. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, and p-p38 in hippocampal tissue and Bcl-2, cleaved caspase-3, and Bax in cortical tissues was observed by Western blot and immunohistochemistry assay, respectively. After GAA treatment, apparent seizure-like EEG with significant arrhythmic disorder and spike waves was reduced or disappeared, and wave amplitude of EEG was reduced significantly. GAA showed similar effect with sodium valproate treatments on epilepsy. There were an apparent improvement of the epileptic behavior and a significant increase in the epileptic latency and shortening of the epileptic duration in the treatment group compared to control. GAA treatment ameliorated the nuclear pyknosis of neurons which appeared seriously in the epilepsy group. GAA treatment significantly reduced the cortical neuron apoptosis of epilepsy and the expression of calcium-sensing receptor, p-P38, p-JNK, cleaved caspase-3, and Bax but increased the expression of both p-ERK and Bcl-2. In conclusion, GAA treatment showed strong antiepileptic effect by decreasing apoptosis in cortical neuron and the expression of calcium-sensing receptor and stimulating the MAPK pathway.Citation
Pang W, Lu S, Zheng R, Li X, Yang S, Feng Y, Wang S, Guo J, Zhou S (2022) 'Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole', BioMed Research International, 22022 (5940372)Publisher
HindawiJournal
BioMed Research InternationalPubMed ID
36093409PubMed Central ID
PMC9458365Additional Links
https://www.hindawi.com/journals/bmri/2022/5940372/Type
ArticleLanguage
enISSN
2314-6133ae974a485f413a2113503eed53cd6c53
10.1155/2022/5940372
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