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dc.contributor.authorHu, Xiao
dc.contributor.authorLiu, Jing
dc.contributor.authorLi, Jun
dc.contributor.authorSong, Yuqiong
dc.contributor.authorChen, Shengjun
dc.contributor.authorZhou, Shaobo
dc.contributor.authorYang, Xianqing
dc.contributor.illustrator
dc.date.accessioned2022-08-09T12:04:43Z
dc.date.available2022-07-22T00:00:00Z
dc.date.available2022-08-09T12:04:43Z
dc.date.issued2022-07-22
dc.identifier.citationHu X, Liu J, Li J, Song Y, Chen S, Zhou S, Yang X (2022) 'Preparation, purification, and identification of novel antioxidant peptides derived from Gracilariopsis lemaneiformis protein hydrolysates', Frontiers in nutrition, 9 (971419)en_US
dc.identifier.issn2296-861X
dc.identifier.pmid35938124
dc.identifier.doi10.3389/fnut.2022.971419
dc.identifier.urihttp://hdl.handle.net/10547/625502
dc.description.abstractGracilariopsis lemaneiformis (G. lemaneiformis) protein was hydrolyzed with alkaline protease to obtain antioxidant peptides. The enzymatic hydrolysis conditions were optimized through single-factor and orthogonal experiments. The results showed that the optimal process parameters were using 2% of alkaline protease, and substrate concentration of 1 g/100 mL and hydrolyzed 2 h at pH 8.0. Gel filtration chromatography and RP-HPLC were adopted for isolating and purifying the antioxidant peptides from the G. lemaneiformis protein hydrolysate (GLPH). Three novel antioxidant peptides were identified as LSPGEL (614.68 Da), VYFDR (698.76 Da), and PGPTY (533.57 Da) by nano-HPLC-MS/MS. The results of ABTS free radical scavenging rate demonstrated PGPTY exhibited the best antioxidant activity (IC50 = 0.24 mg/mL). Moreover, LSPGEL, VYFDR, and PGPTY were docked with Keap1, respectively. The molecular docking results suggested PGPTY had smaller docking energy and inhibition constants than the other two peptides. Finally, the cell viability assay evidenced the protective effect exerted by the antioxidant peptide on H2O2-induced oxidative damage. Above findings showed the potential of using antioxidant peptides from GLPH as antioxidants.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fnut.2022.971419/fullen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGracilariopsis lemaneiformisen_US
dc.subjecthydrolysisen_US
dc.subjectantioxidant peptideen_US
dc.subjectpreparationen_US
dc.subjectpurificationen_US
dc.subjectstructural identificationen_US
dc.subjectSubject Categories::B400 Nutritionen_US
dc.titlePreparation, purification, and identification of novel antioxidant peptides derived from Gracilariopsis lemaneiformis protein hydrolysatesen_US
dc.typeArticleen_US
dc.contributor.departmentChinese Academy of Fishery Sciencesen_US
dc.contributor.departmentJiangsu Ocean Universityen_US
dc.contributor.departmentChinese Academy of Sciencesen_US
dc.contributor.departmentShanghai Ocean Universityen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.contributor.departmentDalian Polytechnic Universityen_US
dc.identifier.journalFrontiers in nutritionen_US
dc.identifier.pmcidPMC9355161
dc.date.updated2022-08-09T11:57:33Z
dc.description.notegold open access
dc.description.fundingThis work was supported by the Key-Area Research and Development Program of Guangdong Province (2020B1111030004), the Guangdong Basic and Applied Basic Research Foundation (2019A1515011588), the China Agriculture Research System of MOF and MARA (CARS-50), the Special Scientific Research Funds for Central Non-profit Institutes, the Chinese Academy of Fishery Sciences (2020TD69), and the Central Public-interest Scientific Institution Basal Research Funds, South China Sea Fisheries Research Institute, CAFS (2021SD06).


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International