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dc.contributor.authorCauz-Santos, Luiz A.
dc.contributor.authorDodsworth, Steven
dc.contributor.authorSamuel, Rosabelle
dc.contributor.authorChristenhusz, Maarten J.M.
dc.contributor.authorPatel, Denise
dc.contributor.authorShittu, Taiwo Adewale
dc.contributor.authorJakob, Aljaž
dc.contributor.authorPaun, Ovidiu
dc.contributor.authorChase, Mark W.
dc.contributor.illustrator
dc.date.accessioned2022-05-16T09:11:07Z
dc.date.available2022-05-10T00:00:00Z
dc.date.available2022-05-16T09:11:07Z
dc.date.issued2022-05-10
dc.identifier.citationCauz-Santos LA, Dodsworth S, Samuel R, Christenhusz MJM, Patel D, Shittu T, Jakob A, Paun O, Chase MW (2022) 'Genomic insights into recent species divergence in Nicotiana benthamiana and natural variation in Rdr1 gene controlling viral susceptibility.', Plant Journal, 111 (1), pp.7-18.en_US
dc.identifier.issn0960-7412
dc.identifier.pmid35535507
dc.identifier.doi10.1111/tpj.15801
dc.identifier.urihttp://hdl.handle.net/10547/625393
dc.description.abstractOne of the most commonly encountered and frequently cited laboratory organisms worldwide is classified taxonomically as Nicotiana benthamiana (Solanaceae), an accession of which, typically referred to as LAB, is renowned for its unique susceptibility to a wide range of plant viruses and hence capacity to be transformed using a variety of methods. This susceptibility is the result of an insertion and consequent loss of function in the RNA dependent RNA polymerase 1 (Rdr1) gene. However, the origin and age of LAB and evolution of N. benthamiana across its wide distribution in Australia remains relatively underexplored. Here, we have used multispecies coalescent methods on genome-wide single nuclear polymorphisms (SNPs) to assess species limits, phylogenetic relationships and divergence times within N. benthamiana. Our results show that the previous taxonomic concept of this species in fact comprises five geographically, morphologically and genetically distinct species, one of which includes LAB. We provide clear evidence that LAB is closely related to accessions collected further north in the Northern Territory; this species split much earlier, c. 1.1 million years ago, from their common ancestor than the other four in this clade and is morphologically the most distinctive. We also found that the Rdr1 gene insertion is variable among accessions from the northern portions of the Northern Territory. Furthermore, this long-isolated species typically grows in sheltered sites in subtropical/tropical monsoon areas of northern Australia, contradicting the previously advanced hypothesis that this species is an extremophile that has traded viral resistance for precocious development.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1111/tpj.15801en_US
dc.rightsYellow - can archive pre-print (ie pre-refereeing)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectgenomicsen_US
dc.subjectNicotiana benthamianaen_US
dc.subjectRdr1en_US
dc.subjectviral susceptibilityen_US
dc.subjectSubject Categories::C410 Applied Geneticsen_US
dc.titleGenomic insights into recent species divergence in Nicotiana benthamiana and natural variation in Rdr1 gene controlling viral susceptibility.en_US
dc.typeArticleen_US
dc.identifier.eissn1365-313X
dc.contributor.departmentUniversity of Viennaen_US
dc.contributor.departmentUniversity of Portsmouthen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.contributor.departmentRoyal Botanic Gardens, Kewen_US
dc.contributor.departmentCurtin Universityen_US
dc.identifier.journalPlant Journalen_US
dc.date.updated2022-05-16T09:05:48Z
dc.description.notegold open access accepted version initially uploaded as that was what was on publisher site first - can add final version when available RVO 16/5/22


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