Genomic insights into recent species divergence in Nicotiana benthamiana and natural variation in Rdr1 gene controlling viral susceptibility.
AuthorsCauz-Santos, Luiz A.
Christenhusz, Maarten J.M.
Shittu, Taiwo Adewale
Chase, Mark W.
AffiliationUniversity of Vienna
University of Portsmouth
University of Bedfordshire
Royal Botanic Gardens, Kew
Subject Categories::C410 Applied Genetics
MetadataShow full item record
AbstractOne of the most commonly encountered and frequently cited laboratory organisms worldwide is classified taxonomically as Nicotiana benthamiana (Solanaceae), an accession of which, typically referred to as LAB, is renowned for its unique susceptibility to a wide range of plant viruses and hence capacity to be transformed using a variety of methods. This susceptibility is the result of an insertion and consequent loss of function in the RNA dependent RNA polymerase 1 (Rdr1) gene. However, the origin and age of LAB and evolution of N. benthamiana across its wide distribution in Australia remains relatively underexplored. Here, we have used multispecies coalescent methods on genome-wide single nuclear polymorphisms (SNPs) to assess species limits, phylogenetic relationships and divergence times within N. benthamiana. Our results show that the previous taxonomic concept of this species in fact comprises five geographically, morphologically and genetically distinct species, one of which includes LAB. We provide clear evidence that LAB is closely related to accessions collected further north in the Northern Territory; this species split much earlier, c. 1.1 million years ago, from their common ancestor than the other four in this clade and is morphologically the most distinctive. We also found that the Rdr1 gene insertion is variable among accessions from the northern portions of the Northern Territory. Furthermore, this long-isolated species typically grows in sheltered sites in subtropical/tropical monsoon areas of northern Australia, contradicting the previously advanced hypothesis that this species is an extremophile that has traded viral resistance for precocious development.
CitationCauz-Santos LA, Dodsworth S, Samuel R, Christenhusz MJM, Patel D, Shittu T, Jakob A, Paun O, Chase MW (2022) 'Genomic insights into recent species divergence in Nicotiana benthamiana and natural variation in Rdr1 gene controlling viral susceptibility.', Plant Journal, (), pp.-.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Yellow - can archive pre-print (ie pre-refereeing)
- Differential Symptom Development and Viral RNA Loads in 10 <i>Nicotiana benthamiana</i> Accessions Infected with the Tobamovirus Yellow Tailflower Mild Mottle Virus.
- Authors: Xu W, Guo Y, Li H, Sivasithamparam K, Jones MGK, Chen X, Wylie SJ
- Issue date: 2022 Mar
- The extremophile Nicotiana benthamiana has traded viral defence for early vigour.
- Authors: Bally J, Nakasugi K, Jia F, Jung H, Ho SY, Wong M, Paul CM, Naim F, Wood CC, Crowhurst RN, Hellens RP, Dale JL, Waterhouse PM
- Issue date: 2015 Nov 2
- Differential responses to virus challenge of laboratory and wild accessions of australian species of nicotiana, and comparative analysis of RDR1 gene sequences.
- Authors: Wylie SJ, Zhang C, Long V, Roossinck MJ, Koh SH, Jones MG, Iqbal S, Li H
- Issue date: 2015
- RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana.
- Authors: Ying XB, Dong L, Zhu H, Duan CG, Du QS, Lv DQ, Fang YY, Garcia JA, Fang RX, Guo HS
- Issue date: 2010 Apr