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dc.contributor.authorYounis, Sidra
dc.contributor.authorDeeba, Farah
dc.contributor.authorFatima Saeed, Rida
dc.contributor.authorMothana, Ramzi A.
dc.contributor.authorUllah, Riaz
dc.contributor.authorFaheem, Muhammad
dc.contributor.authorJaved, Qamar
dc.contributor.authorBlumenberg, Miroslav
dc.identifier.citationYounis S, Deeba F, Fatima Saeed R, Mothana RA, Ullah R, Faheem M, Javed Q, Blumenberg M (2022) 'Regulation of cell cycle and differentiation markers by pathogenic, non-pathogenic and opportunistic skin bacteria', Saudi Journal of Biological Sciences, 29 (3), pp.1717-1729.en_US
dc.description.abstractSkin is the first line of defense against the physical, chemical and the biological environment. It is an ideal organ for studying molecular responses to biological infections through a variety of skin cells that specialize in immune responses. Comparative analysis of skin response to pathogenic, non-pathogenic, and commensal bacteria would help in the identification of disease specific pathways for drug targets. In this study, we investigated human breast reduction skin responses to Cutibacterium acnes (C. acnes), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and TLR1/2 agonist using Affymetrix microarray chips. The Pam3CSK4 solution and bacterial cultures were prepared and inoculated in steel rings, that were placed on the acetone treated epidermis in a petri dish. After 24 h incubation, 8 mm punch biopsies were taken from the center of the ring, and RNA was extracted. The genome-wide expression was then analyzed using Affymetrix HG-133A gene chip microarray. We found that the C. acnes and S. aureus boosted the production of extracellular matrix components and attenuated the expression of differentiation markers. The above responses were mediated through the TLR2 pathway. Skin also responded to S. aureus and C. acnes by inducing the genes of the cell cycle machinery; this response was not TLR2-dependent. S. aureus induced, whereas C. acnes suppressed the genes associated with apoptosis; this was also not TLR2-dependent. Moreover, S. epidermis apparently did not lead to changes in gene expression. We conclude that the breast reduction skin is a very useful model to study the global gene expression in response to bacterial treatments.en_US
dc.description.sponsorshipAuthors wish to thanks researchers supporting Project Number (RSP; 2021/119) at King Saud University Riyadh Saudi Arabia for their financial support. Author also wish to thanks the Higher Edu cation Comisssion Pakistan, Grant/Award Number: PhD Fellowship for 5000; BM7-092” for financial funding the project and Dr Blu menberg who is supported by the R. O. Perelman Department of Dermatology, NYU School of Medicine.en_US
dc.publisherElsevier B.V.en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectacne vulgarisen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectCutibacterium acnesen_US
dc.subjectStaphylococcus epidermidisen_US
dc.subjectSubject Categories::C510 Applied Microbiologyen_US
dc.titleRegulation of cell cycle and differentiation markers by pathogenic, non-pathogenic and opportunistic skin bacteriaen_US
dc.contributor.departmentNational University of Medical Sciences, Pakistanen_US
dc.contributor.departmentQuaid-e-Azam Universityen_US
dc.contributor.departmentNew York Universityen_US
dc.contributor.departmentThe Women University, Pakistanen_US
dc.contributor.departmentKing Saud Universityen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalSaudi Journal of Biological Sciencesen_US
dc.description.notegold open access

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