Effects and mechanism of Ganoderma lucidum polysaccharides in the treatment of diabetic nephropathy in streptozotocin-induced diabetic rats
Authors
Hu, YuWang, Shu-Xiang
Wu, Fu-Yu
Wu, Ke-Jia
Shi, Rui-Ping
Qin, Li-Hong
Lu, Chun-Feng
Wang, Shu-Qiu
Wang, Fang-Fang
Zhou, Shaobo
Issue Date
2022-03-08Subjects
Ganoderma lucidum polysaccharidesdiabetes mellitus
Subject Categories::C131 Applied Cell Biology
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Show full item recordAbstract
Ganoderma lucidum polysaccharides (GLP) have renal protection effect but there was no study on the diabetic nephropathy. This study was designed to investigate its effect and mechanism using a diabetic rat model induced by streptozotocin (50 mg/kg, i.p.). The diabetic rats were treated with GLP (300 mg/kg/day) for 10 weeks. The blood glucose, glycated hemoglobin, body weight, and the levels of blood creatinine, urea nitrogen, and urine protein were assessed. And renal pathologies were assessed by the tissue sections stained with hematoxylin-eosin, Masson’s trichome, and periodic acid-Schiff. The expression of phosphorylated phosphoinositide 3 kinase (p-PI3K), phosphorylated protein kinase B (p-Akt), and phosphorylated mammalian target of rapamycin (p-mTOR), the autophagy proteins beclin-1, LC3-II, LC3-I, and P62; the apoptosis-related proteins caspase-3 and caspase-9; and the inflammation markers IL-6, IL-1β, and TNF-ɑ were assessed. Results showed that GLP alleviated the impairment of renal function by reducing urinary protein excretion and the blood creatinine level and ameliorated diabetic nephropathy. The expression of p-PI3K, p-Akt, and p-mTOR in the diabetic kidney were significantly reduced in the GLP treatment group compared to the without treatment group. GLP treatment activated the autophagy indicators of beclin-1 and the ratio of LC3-II/LC3-I but reduced p62 and also inhibited the expression of caspase-3, caspase-9 and IL-6, IL-1β, and TNF-ɑ. In conclusion, the effect of GLP amelioration diabetic nephropathy may be via the PI3k/Akt/mTOR signaling pathway by inhibition of the apoptosis and inflammation and activation of the autophagy process.Citation
Hu Y, Wang S-X, Wu F-Y, Wu K-J, Shi R-P, Qin L-H, Lu C-F, Wang S-Q, Wang F-F, Zhou S (2022) 'Effects and mechanism of Ganoderma lucidum polysaccharides in the treatment of diabetic nephropathy in streptozotocin-induced diabetic rats', BioMed Research International, 2022 (4314415)Publisher
HindawiJournal
BioMed Research InternationalPubMed ID
35299891PubMed Central ID
PMC8923773Additional Links
https://www.hindawi.com/journals/bmri/2022/4314415/Type
ArticleLanguage
enISSN
2314-6133ae974a485f413a2113503eed53cd6c53
10.1155/2022/4314415
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