A dynamic feedback algorithm of AFM based on cell morphology changes
atomic force microscopy
living cell imaging
Subject Categories::J990 Technologies not elsewhere classified
MetadataShow full item record
AbstractThe atomic force microscope (AFM) used in biological research as a powerful tool has been for many years. However, the imaging of living cells is still a problem as the sample is too high and too soft to obtain their accurate morphologies. Especially in the high-speed scanning mode, AFM does not have enough judgments to make accurate measurements at the down part of the sample. In this study, we propose an improved control method to improve the image quality of living human colon cancer cells (SW480) especially in the down part during the scanning.
CitationCheng C, Wang X, Dong J, Liu Z, Wang Z (2021) 'A dynamic feedback algorithm of AFM based on cell morphology changes', 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Xi'an, China, IEEE.
TypeConference papers, meetings and proceedings
SponsorsThis work was supported by National Key R&D Program of China (No. 2017YFE0112100), EU H2020 Program (MNR4SCell No. 734174), National Natural Science Foundation Program of China (No. 62175020), Jilin Provincial Science and Technology Program (Nos. 2020C022-1, 20190201287JC, 20190702002GH and 20200901011SF), Jilin Province Education Department Program (Nos. JJKH20210833KJ), and “111” Project of China (No. D17017).
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International