A dynamic feedback algorithm of AFM based on cell morphology changes

Abstract

The atomic force microscope (AFM) used in biological research as a powerful tool has been for many years. However, the imaging of living cells is still a problem as the sample is too high and too soft to obtain their accurate morphologies. Especially in the high-speed scanning mode, AFM does not have enough judgments to make accurate measurements at the down part of the sample. In this study, we propose an improved control method to improve the image quality of living human colon cancer cells (SW480) especially in the down part during the scanning.