Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM
atomic force microscopy
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AbstractExosomes play an important role in the early diagnosis and development of hepatocellular carcinoma (HCC). In the past, researchers mainly studied the contents of exosomes and the biological mechanism on cells, but the changes of mechanical properties of cells caused by exosomes are not clear. In this study, the changes of mechanical properties of hepatocytes (L-02) co-cultured with exosomes (7721-exos) derived from HCC (SMMC-7721) with low metastatic ability were studied. The 7721-exos were able to promote the proliferation and rearrange the cytoskeleton of L-02 cells. The atomic force microscopy measurement results showed that the height was increased, the adhesion and the elastic modulus were reduced. The effect was concentration dependent. The changes of cell mechanical properties induced by exosomes were of great significance for further studying the mechanism of exosomes.
CitationJu T, Yang F, Wang Y, Song Z, Xu H, Chen Y, Wang Z (2021) 'Effect of SMMC-7721-derived exosomes on hepatocytes studied by AFM', 2021 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Xi'an, China, IEEE.
TypeConference papers, meetings and proceedings
SponsorsThis work was supported by National Key R&D Program of China (No. 2017YFE0112100), EU H2020 Program (MNR4SCell No. 734174), National Natural Science Foundation Program of China (No. 62175020), Jilin Provincial Science and Technology Program (Nos. 2020C022-1, 20190201287JC, 20190702002GH and 20200901011SF), Jilin Province Education Department Program (No. JJKH20210833KJ), and “111” Project of China (No. D17017). It was partly supported by Changli Nano Biotechnology (China).
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