Optimized degradation and inhibition of α-glucosidase activity by gracilaria lemaneiformis polysaccharide and its production in vitro
Authors
Long, XiaoshanHu, Xiao
Zhou, Shaobo
Xiang, Huan
Chen, Shengjun
Li, Laihao
Liu, Shucheng
Yang, Xianqing
Affiliation
Chinese Academy of Fishery SciencesJiangsu Ocean University
Guangdong Ocean University
University of Bedfordshire
Collaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing
Issue Date
2021-12-22Subjects
polysaccharideα-glucosidase
Gracilaria lemaneiformis
degradation optimization
hypolipidemic activity
chemical characteristics
Subject Categories::D435 Aquaculture
Metadata
Show full item recordAbstract
Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded. After its degradation by hydrogen peroxide combined with vitamin C (H2 O2-Vc) and optimized by Box–Behnken Design (BBD), a new product of GLP-HV will be generated. While using GLP as control, two products of GLP-H (H2 O2-treated) and GLP-V (Vc-treated) were also produced. These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed. The results showed that the optimal conditions for H2 O2-Vc degradation were as follows: H2 O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56◦ C. The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose. The SEM analysis demonstrated that H2 O2-Vc made the structure loose and broken. Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively. GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive. It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.Citation
Long X, Hu X, Zhou S, Xiang H, Chen S, Li L, Liu S, Yang X (2022) 'Optimized degradation and inhibition of α-glucosidase activity by gracilaria lemaneiformis polysaccharide and its production in vitro', Marine Drugs, 20 (1), pp.13-.Publisher
MDPIJournal
Marine DrugsPubMed ID
35049867PubMed Central ID
PMC8777738Additional Links
https://www.mdpi.com/1660-3397/20/1/13Type
ArticleLanguage
enEISSN
1660-3397ae974a485f413a2113503eed53cd6c53
10.3390/md20010013
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