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dc.contributor.authorDong, Lei
dc.contributor.authorJiang, Zhiqiang
dc.contributor.authorYang, Lili
dc.contributor.authorHu, Fen
dc.contributor.authorZheng, Weiwei
dc.contributor.authorXue, Peng
dc.contributor.authorJiang, Songhui
dc.contributor.authorAndersen, Melvin E.
dc.contributor.authorCrabbe, M. James C.
dc.contributor.authorQu, Weidong
dc.contributor.authorHe, Gengsheng
dc.date.accessioned2022-01-04T09:39:00Z
dc.date.available2022-12-07T00:00:00Z
dc.date.available2022-01-04T09:39:00Z
dc.date.issued2021-12-07
dc.identifier.citationDong L, Jiang Z, Yang L, Hu F, Zheng W, Xue P, Jiang S, Andersen ME, He G, Crabbe MJC, Qu W (2022) 'The genotoxic potential of mixed nitrosamines in drinking water involves oxidative stress and Nrf2 activation', Journal of Hazardous Materials, 426 (March 2022), 128010.en_US
dc.identifier.issn0304-3894
dc.identifier.pmid34929594
dc.identifier.doi10.1016/j.jhazmat.2021.128010
dc.identifier.urihttp://hdl.handle.net/10547/625279
dc.description.abstractNitrosamine by-products in drinking water are designated as probable human carcinogens by the IARC, but the health effects of simultaneous exposure to multiple nitrosamines in drinking water remain unknown. Genotoxicity assays were used to assess the effects of both individual and mixed nitrosamines in finished drinking water produced by a large water treatment plant in Shanghai, China. Cytotoxicity and genotoxicity were measured at 1, 10-, 100- and 1000-fold actual concentrations by the Ames test, Comet assay, γ-H2AX assay, and the cytokinesisblock micronuclei assay; oxidative stress and the Nrf2 pathway were also assessed. Nitrosamines detected in drinking water included NDMA (36.45 ng/L), NDPA (44.68 ng/L), and NEMA (37.27 ng/L). Treatment with a mixture of the three nitrosamines at 1000-fold actual drinking-water concentration induced a doubling of revertants in Salmonella typhimurium strain TA100, DNA and chromosome damage in HepG2 cells, while 1–1000-fold concentrations of compounds applied singly lacked these effects. Treatment with 100- and 1000-fold concentrations increased ROS, GSH, and MDA and decreased SOD activity. Thus, nitrosamine mixtures showed greater genotoxic potential than that of the individual compounds. N-Acetylcysteine protected against the nitrosamine-induced chromosome damage, and Nrf2 pathway activation suggested that oxidative stress played pivotal roles in the genotoxic property of the nitrosamine mixtures.en_US
dc.description.sponsorshipThis project was supported by the National Natural Science Foundation of China (81630088, 81930094, 81273035, & 81325017), Changjiang Scholars Program, Ministry of Education (T2014089), Key Project of National High-tech R&D Program of China Ministry of Science and Technology of the People’s Republic of China (No. 2017YFC1600500 & 2013AA065204), National Key R&D Program of China of and a Non-profit Foundation of National Health Ministry in the 12th Five Year Plan (201002001 & 2012BAJ25B05).en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.urlhttps://www.sciencedirect.com/science/article/abs/pii/S0304389421029794en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectgeneticsen_US
dc.subjecthealthen_US
dc.subjectcommunity health servicesen_US
dc.subjectnitrosaminesen_US
dc.subjectdisinfection by-productsen_US
dc.subjectmixed exposureen_US
dc.subjectgenotoxicityen_US
dc.subjectSubject Categories::H122 Water Quality Controlen_US
dc.titleThe genotoxic potential of mixed nitrosamines in drinking water involves oxidative stress and Nrf2 activationen_US
dc.typeArticleen_US
dc.contributor.departmentFudan Universityen_US
dc.contributor.departmentOxford Universityen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalJournal of Hazardous Materialsen_US
dc.date.updated2022-01-04T09:26:55Z
dc.description.note12m embargo


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