Hsp72 and Hsp90α mRNA transcription is characterised by large, sustained changes in core temperature during heat acclimation
Affiliation
Brunel UniversityUniversity of Brighton
University of Bedfordshire
Qatar Orthopaedic and Sports Medicine Hospital
Loughborough University
Issue Date
2016-08-11Subjects
hyperthermiacore temperature
heat acclimation
heat shock proteins
thermotolerance
Subject Categories::C600 Sports Science
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Show full item recordAbstract
Increased intracellular heat shock protein-72 (Hsp72) and heat shock protein-90α (Hsp90α) have been implicated as important components of acquired thermotolerance, providing cytoprotection during stress. This experiment determined the physiological responses characterising increases in Hsp72 and Hsp90α mRNA on the first and tenth day of 90-min heat acclimation (in 40.2 °C, 41.0 % relative humidity (RH)) or equivalent normothermic training (in 20 °C, 29 % RH). Pearson’s product-moment correlation and stepwise multiple regression were performed to determine relationships between physiological [e.g. (Trec, sweat rate (SR) and heart rate (HR)] and training variables (exercise duration, exercise intensity, work done), and the leukocyte Hsp72 and Hsp90α mRNA responses via reverse transcription quantitative polymerase chain reaction (RT-QPCR) (n = 15). Significant (p < 0.05) correlations existed between increased Hsp72 and Hsp90α mRNA (r = 0.879). Increased core temperature was the most important criteria for gene transcription with ΔTrec (r = 0.714), SR (r = 0.709), Trecfinal45 (r = 0.682), area under the curve where Trec ≥ 38.5 °C (AUC38.5 °C; r = 0.678), peak Trec (r = 0.661), duration Trec ≥ 38.5 °C (r = 0.650) and ΔHR (r = 0.511) each demonstrating a significant (p < 0.05) correlation with the increase in Hsp72 mRNA. The Trec AUC38.5 °C (r = 0.729), ΔTrec (r = 0.691), peak Trec (r = 0.680), Trecfinal45 (r = 0.678), SR (r = 0.660), duration Trec ≥ 38.5 °C (r = 0.629), the rate of change in Trec (r = 0.600) and ΔHR (r = 0.531) were the strongest correlate with the increase in Hsp90α mRNA. Multiple regression improved the model for Hsp90α mRNA only, when Trec AUC38.5 °C and SR were combined. Training variables showed insignificant (p > 0.05) weak (r < 0.300) relationships with Hsp72 and Hsp90α mRNA. Hsp72 and Hsp90α mRNA correlates were comparable on the first and tenth day. When transcription of the related Hsp72 and Hsp90α mRNA is important, protocols should rapidly induce large, prolonged changes in core temperature.Citation
Gibson O, Tuttle J, Watt P, Maxwell N, Taylor L (2016) 'Hsp72 and Hsp90α mRNA transcription is characterised by large, sustained changes in core temperature during heat acclimation', Cell Stress and Chaperones, 21 (6), pp.1021-1035.Publisher
SpringerJournal
Cell Stress and ChaperonesPubMed ID
27511024PubMed Central ID
PMC5083671Additional Links
https://link.springer.com/article/10.1007%2Fs12192-016-0726-0Type
ArticleLanguage
enISSN
1355-8145ae974a485f413a2113503eed53cd6c53
10.1007/s12192-016-0726-0
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