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dc.contributor.authorYan, Jin
dc.contributor.authorXie, Chenchen
dc.contributor.authorZhu, Jiajing
dc.contributor.authorSong, Zhengxun
dc.contributor.authorWang, Zuobin
dc.contributor.authorLi, Li
dc.date.accessioned2021-08-11T10:15:33Z
dc.date.available2022-08-05T00:00:00Z
dc.date.available2021-08-11T10:15:33Z
dc.date.issued2021-08-05
dc.identifier.citationYan J, Xie C, Zhu J, Song Z, Wang Z, Li L (2021) 'Effect of trypsin concentration on living SMCC-7721 cells studied by atomic force microscopy', Journal of Microscopy, 284 (3), pp.203-213.en_US
dc.identifier.issn0022-2720
dc.identifier.pmid34350998
dc.identifier.doi10.1111/jmi.13053
dc.identifier.urihttp://hdl.handle.net/10547/625079
dc.description.abstractTrypsin is playing an important role in the processes of cancer proliferation, invasion, and metastasis which require the precise information of morphology and mechanical properties on the nanoscale for the related research. In this work, living human hepatoma (SMCC-7721) cells were treated with different concentrations of trypsin solution. The morphology and mechanical properties of the cells were measured via atomic force microscope (AFM). Statistical analyses of measurement data indicated that with the increase of trypsin concentration, the average cell height and the surface roughness were both increased, but the cell viability, the cell surface adhesion and the elasticity modulus were decreased significantly. The force required to puncture the cells was also gradually reduced. It indicates that trypsin not only hydrolyzes the proteins between the cell and the substrate but also the membrane proteins. The results offer valuable clues for the cancerous process study, pathological analysis, and trypsin inhibitor drug development. And this work provides an effective way for overcoming the cell membrane in drug injection for cell-targeted therapy. This article is protected by copyright. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1111/jmi.13053en_US
dc.rightsYellow - can archive pre-print (ie pre-refereeing)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectatomic force microscopyen_US
dc.subjecttrypsinen_US
dc.subjectSubject Categories::F360 Optical Physicsen_US
dc.titleEffect of trypsin concentration on living SMCC-7721 cells studied by atomic force microscopyen_US
dc.typeArticleen_US
dc.identifier.eissn1365-2818
dc.identifier.journalJournal of Microscopyen_US
dc.date.updated2021-08-11T10:10:01Z
dc.description.note12m embargo


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