Effect of trypsin concentration on living SMCC-7721 cells studied by atomic force microscopy
Abstract
Trypsin is playing an important role in the processes of cancer proliferation, invasion, and metastasis which require the precise information of morphology and mechanical properties on the nanoscale for the related research. In this work, living human hepatoma (SMCC-7721) cells were treated with different concentrations of trypsin solution. The morphology and mechanical properties of the cells were measured via atomic force microscope (AFM). Statistical analyses of measurement data indicated that with the increase of trypsin concentration, the average cell height and the surface roughness were both increased, but the cell viability, the cell surface adhesion and the elasticity modulus were decreased significantly. The force required to puncture the cells was also gradually reduced. It indicates that trypsin not only hydrolyzes the proteins between the cell and the substrate but also the membrane proteins. The results offer valuable clues for the cancerous process study, pathological analysis, and trypsin inhibitor drug development. And this work provides an effective way for overcoming the cell membrane in drug injection for cell-targeted therapy. This article is protected by copyright. All rights reserved.Citation
Yan J, Xie C, Zhu J, Song Z, Wang Z, Li L (2021) 'Effect of trypsin concentration on living SMCC-7721 cells studied by atomic force microscopy', Journal of Microscopy, 284 (3), pp.203-213.Publisher
WileyJournal
Journal of MicroscopyPubMed ID
34350998Additional Links
https://onlinelibrary.wiley.com/doi/10.1111/jmi.13053Type
ArticleLanguage
enISSN
0022-2720EISSN
1365-2818ae974a485f413a2113503eed53cd6c53
10.1111/jmi.13053
Scopus Count
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- Creative Commons
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