Evaluation of genetic diversity and population structure of Fragaria nilgerrensis using EST-SSR markers
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Authors
Liu, JieZhang, Yichen
Diao, Xia
Yu, Kun
Dai, Xiongwei
Qu, Peng
Crabbe, M. James C.
Zhang, Ti-Cao
Qiao, Qin
Affiliation
Yunnan UniversityOxford University
Shanxi University
University of Bedfordshire
Yunnan University of Chinese Medicine
Issue Date
2021-06-25Subjects
geneticsplants
Fragaria nilgerrensis
EST-SSR marker
genetic diversity
population structure
Subject Categories::C410 Applied Genetics
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Fragaria nilgerrensis is a diploid wild strawberry widely distributed in Southwest China. Its white color and “peach-like” fragrance of fruits are valuable characters for the genetic improvement of cultivated strawberry plants. Its strong biotic and abiotic resistance and tolerance also enable it to survive in different habitats in the field. In this study, we evaluated the level of genetic variation within and between 16 populations with 169 individuals of F. nilgerrensis using 16 newly developed EST-SSR (expressed sequence tag-simple sequence repeats) markers. The results show that the genetic diversity of this species was high, based on Nei’s genetic diversity (0.26) and polymorphic loci (0.41), although it is self-compatible and has clonal propagation. Significant genetic differentiation among populations was also detected by AMOVA analysis (Fst = 0.34), which could be indicative of little gene flow (Nm = 0.43) in F. nilgerrensis. The phylogenetic tree indicates that most of individuals from the same population have clustered together. These populations were not grouped based on the geographical distance, consistent with the Mantel test result (R2 = 0.0063, P > 0.05). All the populations were assigned into two ancestral groups, with some individuals admixed, suggesting ancestral gene flow had occurred between these two groups. Our developed EST-SSR markers as well as the genetic diversity and population structure analysis of F. nilgerrensis are important for genetic improvement in the breeding process. Moreover, the populations that contain high genetic diversity would be a priority for collection and conservation.Citation
Liu J., Zhang Y, Diao X, Yu K, Dai X, Qu P, Crabbe MJC, Zhang T, Qiao Q (2021) 'Evaluation of genetic diversity and population structure of Fragaria nilgerrensis using EST-SSR markers', Gene, 796-797 (145791)Publisher
ElsevierJournal
GenePubMed ID
34175390Type
ArticleLanguage
enISSN
0378-1119ae974a485f413a2113503eed53cd6c53
10.1016/j.gene.2021.145791
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