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dc.contributor.authorCosta, Lucas
dc.contributor.authorMarques, André
dc.contributor.authorBuddenhagen, Chris
dc.contributor.authorThomas, William Wayt
dc.contributor.authorHuettel, Bruno
dc.contributor.authorSchubert, Veit
dc.contributor.authorDodsworth, Steven
dc.contributor.authorHouben, Andreas
dc.contributor.authorSouza, Gustavo
dc.contributor.authorPedrosa-Harand, Andrea
dc.date.accessioned2021-06-11T10:43:47Z
dc.date.available2021-06-11T10:43:47Z
dc.date.issued2021-05-29
dc.identifier.citationCosta L, Marques A, Buddenhagen C, Thomas WW, Huettel B, Schubert V, Dodsworth S, Houben A, Souza G, Pedrosa-Harand A (2021) 'Aiming off the target: recycling target capture sequencing reads for investigating repetitive DNA', Annals of Botany, (), pp.-.en_US
dc.identifier.issn0305-7364
dc.identifier.pmid34050647
dc.identifier.doi10.1093/aob/mcab063
dc.identifier.urihttp://hdl.handle.net/10547/625011
dc.description.abstractWith the advance of high-throughput sequencing (HTS), reduced-representation methods such as target capture sequencing (TCS) emerged as cost-efficient ways of gathering genomic information, particularly from coding regions. As the off-target reads from such sequencing are expected to be similar to genome skimming (GS), we assessed the quality of repeat characterization in plant genomes using this data. Repeat composition obtained from TCS datasets of five Rhynchospora (Cyperaceae) species were compared with GS data from the same taxa. In addition, a FISH probe was designed based on the most abundant satellite found in the TCS dataset of Rhynchospora cephalotes. Finally, repeat-based phylogenies of the five Rhynchospora species were constructed based on the GS and TCS dataset and the topologies were compared with a gene-alignment based phylogenetic tree. All the major repetitive DNA families were identified in TCS, including repeats that showed abundances as low as 0.01% in the GS data. Rank correlation between GS and TCS repeat abundances were moderately high (r = 0.58-0.85), increasing after filtering out the targeted loci from the raw TCS reads (r = 0.66-0.92). Repeat data obtained by TCS was also reliable to develop a cytogenetic probe of a new variant of the holocentromeric satellite Tyba. Repeat-based phylogenies from TCS data were congruent with those obtained from GS data and the gene-alignment tree. Our results show that off-target TCS reads can be recycled to identify repeats for cyto- and phylogenomic investigations. Given the growing availability of TCS reads, driven by global phylogenomic projects, our strategy represents a way to recycle genomic data and contribute to a better characterization of plant biodiversity.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.urlhttps://academic.oup.com/aob/advance-article-abstract/doi/10.1093/aob/mcab063/6288108en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.subjecthigh-throughput sequencingen_US
dc.subjectrepetitive DNAen_US
dc.titleAiming off the target: recycling target capture sequencing reads for investigating repetitive DNAen_US
dc.typeArticleen_US
dc.identifier.eissn1095-8290
dc.identifier.journalAnnals of Botanyen_US
dc.date.updated2021-06-11T10:39:45Z
dc.description.note


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