The influence of different liquid environments on the atomic force microscopy detection of living bEnd.3 cells
Subjectsatomic force microscopy
MetadataShow full item record
AbstractAtomic force microscopy (AFM) is one of the most important tools in the field of biomedical science, and it can be used to perform the high-resolution three-dimensional imaging of samples in liquid environments to obtain their physical properties (such as surface potentials and mechanical properties). The influence of the liquid environment on the image quality of the sample and the detection results cannot be ignored. In this work, quantitative imaging (QI) mode AFM imaging and mechanical detection were performed on mouse brain microvascular endothelial (bEnd.3) cells in different liquid environments. The gray-level variance product (SMD2) function was used to evaluate the imaging quality of the cells in liquids with different physical properties, and the variations in cell mechanical properties were quantitatively analyzed. An AFM detection liquid containing less ions and organics compared with the traditional culture medium, which is beneficial for improving the imaging quality, is introduced, and it shows similar mechanical detection results within 3 h. This can greatly reduce the detection costs and could have positive significance in the field of AFM living-cell detection.
CitationYan J, Sun B, Xie C, Liu Y, Song Z, Xu H, Wang Z (2021) 'The influence of different liquid environments on the atomic force microscopy detection of living bEnd.3 cells', Analytical Methods, (13), pp.2384-2390.
PublisherRoyal Society of Chemistry
- Imaging in Biologically-Relevant Environments with AFM Using Stiff qPlus Sensors.
- Authors: Pürckhauer K, Weymouth AJ, Pfeffer K, Kullmann L, Mulvihill E, Krahn MP, Müller DJ, Giessibl FJ
- Issue date: 2018 Jun 19
- High-resolution noncontact atomic force microscopy.
- Authors: Pérez R, García R, Schwarz U
- Issue date: 2009 Jul 1
- Nanointerrogation of ultrasonic contrast agent microbubbles using atomic force microscopy.
- Authors: Sboros V, Glynos E, Pye SD, Moran CM, Butler M, Ross J, Short R, McDicken WN, Koutsos V
- Issue date: 2006 Apr
- Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells.
- Authors: Moura CC, Miranda A, Oreffo ROC, De Beule PAA
- Issue date: 2020 Aug 20
- Improvements in fundamental performance of in-liquid frequency modulation atomic force microscopy.
- Authors: Fukuma T
- Issue date: 2020 Dec 3