MicroRNAs for virus pathogenicity and host responses, identified in SARS-CoV-2 genomes, may play roles in viral-host co-evolution in putative zoonotic host species
MetadataShow full item record
AbstractOur recent study identified seven key microRNAs (miR-8066, 5197, 3611, 3934-3p, 1307-3p, 3691-3p, 1468-5p) similar between SARS-CoV-2 and the human genome, pointing at miR-related mechanisms in viral entry and the regulatory effects on host immunity. To identify the putative roles of these miRs in zoonosis, we assessed their conservation, compared with humans, in some key wild and domestic animal carriers of zoonotic viruses, including bat, pangolin, pig, cow, rat, and chicken. Out of the seven miRs under study, miR-3611 was the most strongly conserved across all species; miR-5197 was the most conserved in pangolin, pig, cow, bat, and rat; miR-1307 was most strongly conserved in pangolin, pig, cow, bat, and human; miR-3691-3p in pangolin, cow, and human; miR-3934-3p in pig and cow, followed by pangolin and bat; miR-1468 was most conserved in pangolin, pig, and bat; while miR-8066 was most conserved in pangolin and pig. In humans, miR-3611 and miR-1307 were most conserved, while miR-8066, miR-5197, miR-3334-3p and miR-1468 were least conserved, compared with pangolin, pig, cow, and bat. Furthermore, we identified that changes in the miR-5197 nucleotides between pangolin and human can generate three new miRs, with differing tissue distribution in the brain, lung, intestines, lymph nodes, and muscle, and with different downstream regulatory effects on KEGG pathways. This may be of considerable importance as miR-5197 is localized in the spike protein transcript area of the SARS-CoV-2 genome. Our findings may indicate roles for these miRs in viral-host co-evolution in zoonotic hosts, particularly highlighting pangolin, bat, cow, and pig as putative zoonotic carriers, while highlighting the miRs' roles in KEGG pathways linked to viral pathogenicity and host responses in humans. This in silico study paves the way for investigations into the roles of miRs in zoonotic disease.
CitationLange S, Arisan ED, Grant GH, Uysal-Onganer P (2021) 'MicroRNAs for virus pathogenicity and host responses, identified in SARS-CoV-2 genomes, may play roles in viral-host co-evolution in putative zoonotic host species', Viruses, 13 (1), pp.117-.
PubMed Central IDPMC7830670
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International
- The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities.
- Authors: Arisan ED, Dart A, Grant GH, Arisan S, Cuhadaroglu S, Lange S, Uysal-Onganer P
- Issue date: 2020 Jun 4
- Molecular evolution and phylogenetic analysis of SARS-CoV-2 and hosts ACE2 protein suggest Malayan pangolin as intermediary host.
- Authors: Lopes LR, de Mattos Cardillo G, Paiva PB
- Issue date: 2020 Dec
- Computational Analysis of SARS-CoV-2 and SARS-Like Coronavirus Diversity in Human, Bat and Pangolin Populations.
- Authors: Dimonaco NJ, Salavati M, Shih BB
- Issue date: 2020 Dec 30
- Computational Analysis of Targeting SARS-CoV-2, Viral Entry Proteins ACE2 and TMPRSS2, and Interferon Genes by Host MicroRNAs.
- Authors: Pierce JB, Simion V, Icli B, Pérez-Cremades D, Cheng HS, Feinberg MW
- Issue date: 2020 Nov 16
- The novel coronavirus SARS-CoV-2: From a zoonotic infection to coronavirus disease 2019.
- Authors: Dos Santos Bezerra R, Valença IN, de Cassia Ruy P, Ximenez JPB, da Silva Junior WA, Covas DT, Kashima S, Slavov SN
- Issue date: 2020 Nov