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dc.contributor.authorLi, Yunzhou
dc.contributor.authorYue, Ningbo
dc.contributor.authorBasit, Abdul
dc.contributor.authorLi, Yulong
dc.contributor.authorZhang, Dalong
dc.contributor.authorQin, Lei
dc.contributor.authorCrabbe, M. James C.
dc.contributor.authorXu, Wen
dc.contributor.authorWang, Yong
dc.contributor.authorYan, Jianmin
dc.date.accessioned2021-02-08T09:25:15Z
dc.date.available2021-02-07T00:00:00Z
dc.date.available2021-02-08T09:25:15Z
dc.date.issued2021-01-15
dc.identifier.citationLi Y, Yue N, Basit A, Li Y, Zhang D, Qin L, Crabbe MJC, Xu W, Wang Y, Yan J (2021) 'Targeted editing of SlMAPK6 using CRISPR/Cas9 technology to promote the development of axillary buds in tomato plants', Journal of Agricultural Science, 13 (2), pp.11-22.en_US
dc.identifier.issn1916-9752
dc.identifier.doi10.5539/jas.v13n2p11
dc.identifier.urihttp://hdl.handle.net/10547/624812
dc.description.abstractThe mitogen-activated protein kinase (MAPK) cascade signaling system has been relatively conserved throughout the evolution of eukaryotes and is involved in the regulation of growth and development and metabolism. In this study, dwarf tomato plants were used as the research material. First, the tissue-specific expression of SlMAPK6 was measured in wild-type plants by quantitative RT-PCR. The results showed that SlMAPK6 was highly expressed in the tissues of the stems, leaves and flowers but was expressed at low levels in the tissues of the roots, sepals and fruits. Second, SlMAPK6-knockout lines CRISPR-3 and CRISPR-7 were obtained by CRISPR-Cas9 technology and Agrobacterium-mediated transformation. Compared with wild-type, the mutant lines CRISPR-3 and CRISPR-7 showed significant phenotypic characteristics, such as increased numbers of axillary buds and true leaves, thickened stems, and longer leaflets. In addition, to explore the molecular mechanism by which MAPK regulates axillary bud growth, we also showed that SlMAPK6 positively regulates the strigolactone synthesis genes SlCCD7 and SlCCD8 and the gibberellin (GA) synthesis genes GA20ox3 and GA3ox1 and negatively regulates the axillary bud development-related genes Ls, BL and BRC1b/TCP8 and the GA synthesis inhibitory gene GAI. Therefore, SlMAPK6 appears to regulate the synthesis of strigolactone and GA to induce the growth and development of tomato axillary buds.en_US
dc.description.sponsorshipNational Natural Science Foundation of China (No. 32060679; No.31760594; No. 31960604), Research Project on Talent Introduction of Guizhou University (No. [2017]50) and National Natural Fund Cultivation Project of Guizhou University (No. [2017] 5788-28).en_US
dc.language.isoenen_US
dc.publisherCanadian Center of Science and Educationen_US
dc.relation.urlhttp://www.ccsenet.org/journal/index.php/jas/article/view/0/44575en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectgeneticsen_US
dc.subjectflowering plantsen_US
dc.subjectcell signallingen_US
dc.subjectSubject Categories::C240 Plant Cell Scienceen_US
dc.titleTargeted editing of SlMAPK6 using CRISPR/Cas9 technology to promote the development of axillary buds in tomato plantsen_US
dc.typeArticleen_US
dc.identifier.eissn1916-9760
dc.contributor.departmentGuizhou Universityen_US
dc.contributor.departmentNorthwest A&F Universityen_US
dc.contributor.departmentShandong Agricultural Universityen_US
dc.contributor.departmentNortheast Agricultural Universityen_US
dc.contributor.departmentOxford Universityen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalJournal of Agricultural Scienceen_US
dc.date.updated2021-02-08T09:18:46Z
dc.description.noteopen access with cclicence


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