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    A study on the effects of tumor-derived exosomes on hepatoma cells and hepatocytes by atomic force microscopy

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    Authors
    Ju, Tuoyu
    Wang, Shuwei
    Wang, Jiajia
    Yang, Fan
    Song, Zhengxun
    Xu, Hongmei
    Chen, Yujuan
    Zhang, Jingran
    Wang, Zuobin
    Affiliation
    Changchun University of Science and Technology
    Jilin University
    University of Bedfordshire
    Issue Date
    2020-12-07
    Subjects
    atomic force microscopy
    
    Metadata
    Show full item record
    Abstract
    Tumor-derived exosomes (exos) are closely related to the occurrence, development and treatment of tumors. However, it is not clear how the exosomes affect the physical properties, which lead to the deterioration of the target cells. In this paper, atomic force microscopy (AFM) was used to study the effects of exosomes in HCC-LM3 cells and other cells (SMMC-7721 and HL-7702). The results showed that the HCC-LM3-exos (the exosomes secreted by HCC-LM3 cells, 50 μg mL-1) significantly promoted the proliferation and migration of HCC-LM3 cells. HCC-LM3-exos also promoted the proliferation and migration of SMMC-7721 and HL-7702 cells at 1000 and 1500 μg mL-1, respectively. With an increase in time and concentration, the proliferation effect was more significant. On comparing the mechanical properties of the three types of cells (HCC-LM3, SMMC-7721 and HL-7702 cells), the degradation degree and migration ability of the cells were from high to low in the above order. In turn, the surface roughness of the cells decreased, and adhesion and elastic modulus increased. With an increase in treatment time, surface roughness increased, while adhesion and elastic modulus decreased. These suggested that the HCC-LM3-exos could change the mechanical properties of cells, leading to their deterioration, and enhance their migration and invasion ability. In this paper, the effects of exosomes were analyzed from the perspective of the physical parameters of cells, which provide a new idea to study cancer metastasis and prognosis.
    Citation
    Ju T, Wang S, Wang J, Yang F, Song Z, Xu H, Chen Y, Zhang J, Wang Z (2020) 'A study on the effects of tumor-derived exosomes on hepatoma cells and hepatocytes by atomic force microscopy', Analytical Methods, 12 (45), pp.5458-5467.
    Publisher
    Royal Society of Chemistry
    Journal
    Analytical Methods
    URI
    http://hdl.handle.net/10547/624732
    DOI
    10.1039/d0ay01730b
    Additional Links
    https://pubs.rsc.org/en/content/articlelanding/2020/AY/D0AY01730B#!divAbstract
    Type
    Article
    Language
    en
    ISSN
    1759-9660
    ae974a485f413a2113503eed53cd6c53
    10.1039/d0ay01730b
    Scopus Count
    Collections
    Computing

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