Downhill running and exercise in hot environments increase leukocyte Hsp72 (HSPA1A) and Hsp90 (HSPC1) gene transcripts
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AbstractStressors within humans and other species activate Hsp72 and Hsp90 mRNA transcription, although it is unclear which environmental temperature or treadmill gradient induces the largest increase. To determine the optimal stressor for priming the Hsp system, physically active but not heat-acclimated participants (19.8 1.9 and 20.9 3.6 yr) exercised at lactate threshold in either temperate (20°C, 50% relative humidity; RH) or hot (30°C, 50% RH) environmental conditions. Within each condition, participants completed a flat running (temperate flat or hot flat) and a downhill running (temperate downhill or hot downhill) experimental trial in a randomized counterbalanced order separated by at least 7 days. Venous blood samples were taken immediately before (basal), immediately after exercise, and 3 and 24 h postexercise. RNA was extracted from leukocytes and RT-quantitative PCR conducted to determine Hsp72 and Hsp90 mRNA relative expression. Leukocyte Hsp72 mRNA was increased immediately after exercise following downhill running (1.9 0.9-fold) compared with flat running (1.3 0.4-fold; P 0.001) and in hot (1.9 0.6-fold) compared with temperate conditions (1.1 0.5-fold; P 0.003). Leukocyte Hsp90 mRNA increased immediately after exercise following downhill running (1.4 0.8-fold) compared with flat running (0.9 0.6-fold; P 0.002) and in hot (1.6 1.0-fold) compared with temperate conditions (0.9 0.6-fold; P 0.003). Downhill running and exercise in hot conditions induced the largest stimuli for leukocyte Hsp72 and Hsp90 mRNA increases.
CitationTuttle JA, Castle PC, Metcalfe AJ, Midgley AW, Taylor L, Lewis MP (2015) 'Downhill running and exercise in hot environments increase leukocyte Hsp72 (HSPA1A) and Hsp90 (HSPC1) gene transcripts', Journal of Applied Physiology, 118 (8), pp.996-1005.
PublisherAmerican Physiological Society
JournalJournal of Applied Physiology
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