Bovine milk fat globule epidermal growth factor Ⅷ activates PI3K/Akt signaling pathway and attenuates sarcopenia in rat model induced by D-galactose
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Bovine+Milk+Fat+Globule+Epider ...
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2021-12-17
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Affiliation
Jiangsu Normal UniversityHarbin Institute of Technology
Northeast Agriculture University
University of Bedfordshire
Issue Date
2020-12-17Subjects
MFG-E8sarcopenic model rat
myoblasts
PI3K/Akt signalling pathway
Subject Categories::C910 Applied Biological Sciences
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To develop a more effective and safer treatment for sarcopenia, this research investigated the anti-sarcopenia mechanism of Milk Fat Globule Epidermal Growth Factor Ⅷ (MFG-E8) from the liver function and metabolism in sarcopenic model rat. After 4 weeks nutritional intervention experiment, MFG-E8 can significantly increase the gastrocnemius mass in rat. The mechanism of MFG-E8 in improving sarcopenia was related to its promotional capacity to the activities of superoxide dismutase (SOD) activity in serum, Glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in liver. Meanwhile, MFG-E8 could also down-regulate obesity-related indicators, such as triglyceride (TG) and Non-esterified fatty acid (NEFA). The analysis of liver and gastrocnemius histopathology found that MFG-E8 could reduce the accumulation of fatty vesicles, improve liver function, thereby alleviating gastrocnemius tissue inflammation. In vitro experiments, myoblasts obtained from gastrocnemius tissue showed that MFG-E8 could reduce mitochondrial autophagy and inhibit cell apoptosis. In addition, MFG-E8 could up-regulate the phosphorylation level of PI3K via activating PI3K/Akt signaling pathway in gastrocnemius tissue, and promote the formation of muscle fibers, thereby increasing muscle mass. Moreover, MFG-E8 could also promote the formation of neuromuscular junctions by up-regulating the mRNA and protein expression of MusK in gastrocnemius.Citation
Li H, Wang R, Wang LF, Li L, Ma Y, Zhou S (2020) 'Bovine milk fat globule epidermal growth factor Ⅷ activates PI3K/Akt signaling pathway and attenuates sarcopenia in rat model induced by D-galactose', Food Bioscience, 40 (100847)Publisher
ElsevierJournal
Food BioscienceAdditional Links
https://www.sciencedirect.com/science/article/pii/S2212429220311858Type
ArticleLanguage
enISSN
2212-4292ae974a485f413a2113503eed53cd6c53
10.1016/j.fbio.2020.100847
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