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dc.contributor.authorShaher, Fahmi
dc.contributor.authorWang, Shuqiu
dc.contributor.authorQiu, Hong-Bin
dc.contributor.authorHu, Yu
dc.contributor.authorZhang, Yu
dc.contributor.authorWang, Weiqun
dc.contributor.authorAL-ward, Hisham
dc.contributor.authorAbdulghani, Mahfoudh A. M.
dc.contributor.authorBaldi, Salem
dc.contributor.authorZhou, Shaobo
dc.identifier.citationShaher J, Wang S, Qiu H, Hu Y, Zhang Y, Wang W, AL-Ward H, Abdulghani MAM, Baldi S, Zhou S (2020) 'Effect and mechanism of Ganoderma lucidum spores on alleviation diabetic cardiomyopathy in a pilot in vivo study ', Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, pp.4809-4822.en_US
dc.description.abstractBackground: Ganoderma lucidum spores (GLS) exhibit disease prevention properties, but no study has been carried out on the anti-diabetic cardiomyopathy property of GLS. The aim of this study is to evaluate the hyperglycemia-mediated cardiomyopathy protection and mechanisms of GLS in diabetic rats induced by streptozotocin (STZ). Methods: Male SD rats were randomly divided into three groups. Two groups were given STZ (50 mg/kg, i.p.) treatment and when their fasting plasma glucose was above 16.7 mmol/L, one group was given placebo, as diabetic group; and another group was given GLS (300 mg/kg) treatment. The group without STZ treatment was given placebo as a control group. The experiment lasted 70 days. The histology of myocardium and biomarkers of antioxidant, myocardial injury, pro-inflammatory cytokines, pro-apoptotic proteins and phosphorylation of key proteins in PI3K/AKT pathway were assessed. Results: Biochemical analysis showed that GLS treatment significantly reduced the blood glucose (-20.3%) and triglyceride (-20.4%) levels compared to diabetic group without treatment. GLS treatment decreased the content of MDA (-25.6%) and activity of lactate dehydrogenase (-18.9%) but increased the activity of GSH-Px (65.4%). Western blot analysis showed that GLS treatment reduced the expression of both alpha-smooth muscle actin and brain natriuretic peptide. Histological analysis on the cardiac tissue micrographs showed that GLS treatment reduced the collagen fibroses and glycogen reactivity in myocardium. Both western blot and immunohistochemistry analyses showed that GLS treatment decreased the expression levels of pro-inflammatory factors (cytokines IL-1β, and TNF-α) as well as apoptosis regulatory proteins (Bax, caspase-3 and -9), but increased the Bcl-2. Moreover, GLS treatment significantly increased the phosphorylation of key proteins involved in PI3K/AKT pathway, e.g. p-AKT p-PI3K and mTOR. Conclusion: The results indicated that GLS treatment alleviates diabetic cardiomyopathy by reducing hyperglycemia, oxidative stress, inflammation, apoptosis and further attenuating the fibrosis and myocardial dysfunction induced by STZ through the stimulation of the PI3K/Akt/mTOR signaling pathway.en_US
dc.publisherDove Pressen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectGanoderma lucidum sporeen_US
dc.subjectPI3K/Akt/mTOR signaling pathwayen_US
dc.subjectdiabetic cardiomyopathyen_US
dc.subjectSubject Categories::C730 Metabolic Biochemistryen_US
dc.titleEffect and mechanism of Ganoderma lucidum spores on alleviation diabetic cardiomyopathy in a pilot in vivo studyen_US
dc.contributor.departmentJiamusi Universityen_US
dc.contributor.departmentQassim Universityen_US
dc.contributor.departmentDalian Medical Universityen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.identifier.journalDiabetes, Metabolic Syndrome and Obesity: Targets and Therapyen_US
dc.description.noteopen access

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