Fbp17 and cip4 recruit ship2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis
AuthorsHak, Laura Chan Wah
Meglio, Ilaria Di
Häsler, Safa Lucken-Ardjomande
Quintaneiro, Leonor M.
Ferreira, Antonio P.A.
McMahon, Harvey T.
MetadataShow full item record
AbstractEndocytosis mediates the cellular uptake of micronutrients and the turnover of plasma membrane proteins. Clathrin-mediated endocytosis is the major uptake pathway in resting cells 1 , but several clathrin-independent endocytic routes exist in parallel 2,3 . One such pathway, fast endophilin-mediated endocytosis (FEME), is not constitutive but triggered upon activation of certain receptors, including the β 1 adrenergic receptor 4 . FEME activates promptly following stimulation as endophilin is pre-enriched by the phosphatidylinositol-3,4-bisphosphate-binding protein lamellipodin 4,5 . However, in the absence of stimulation, endophilin foci abort and disassemble after a few seconds. Looking for additional proteins involved in FEME, we found that 20 out of 65 BAR domain-containing proteins tested colocalized with endophilin spots. Among them, FBP17 and CIP4 prime the membrane of resting cells for FEME by recruiting the 5′-lipid phosphatase SHIP2 and lamellipodin to mediate the local production of phosphati-dylinositol-3,4-bisphosphate and endophilin pre-enrichment. Membrane-bound GTP-loaded Cdc42 recruits FBP17 and CIP4, before being locally deactivated by RICH1 and SH3BP1 GTPase-activating proteins. This generates the transient assembly and disassembly of endophilin spots, which lasts 5–10 seconds. This mechanism periodically primes patches of the membrane for prompt responses upon FEME activation.
CitationHak LCW, Khan S, Meglio ID, Law AL, Häsler SLA, Quintaneiro LM, Ferreira APA, Krause M, McMahon HT, Boucrot E (2018) 'Fbp17 and cip4 recruit ship2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis', Nature Cell Biology, 20 (9), pp.1023-1031.
PublisherNature Publishing Group
JournalNature Cell Biology
PubMed Central IDPMC6122583
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- Publisher Correction: FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis.
- Authors: Chan Wah Hak L, Khan S, Di Meglio I, Law AL, Lucken-Ardjomande Häsler S, Quintaneiro LM, Ferreira APA, Krause M, McMahon HT, Boucrot E
- Issue date: 2018 Oct