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dc.contributor.authorJanganan, Thamarai K.
dc.contributor.authorMullin, Nic
dc.contributor.authorDafis-Sagarmendi, Ainhoa
dc.contributor.authorBrunt, Jason
dc.contributor.authorTzokov, Svetomir B.
dc.contributor.authorStringer, Sandra
dc.contributor.authorMoir, Anne
dc.contributor.authorChaudhuri, Roy R.
dc.contributor.authorFagan, Robert P.
dc.contributor.authorHobbs, Jamie K.
dc.contributor.authorBullough, Per A.
dc.date.accessioned2020-11-23T10:06:19Z
dc.date.available2020-07-01T00:00:00Z
dc.date.available2020-11-23T10:06:19Z
dc.date.issued2020-07-01
dc.identifier.citationJanganan TK, Mullin N, Dafis-Sagarmendi A, Brunt J, Tzokov SB, Stringer S, Moir A, Chaudhuri RR, Fagan RP, Hobbs JK, Bullough PA (2020) 'Architecture and self-assembly of Clostridium sporogenes and Clostridium botulinum spore surfaces illustrate a general protective strategy across spore formers', MSPHERE, 5 (4), pp.e00424-2.en_US
dc.identifier.issn2379-5042
dc.identifier.doi10.1128/mSphere.00424-20
dc.identifier.urihttp://hdl.handle.net/10547/624631
dc.description.abstractSpores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective properties through extensive disulfide cross-linking of self-assembled arrays of cysteine-rich proteins. We predicted that this could be a mechanism employed by spore formers in general, even those from other genera. Here, we tested this by revealing in nanometer detail how the outer envelope (exosporium) in Clostridium sporogenes (surrogate for C. botulinum group I), and in other clostridial relatives, forms a hexagonally symmetric semipermeable array. A cysteine-rich protein, CsxA, when expressed in Escherichia coli, self-assembles into a highly thermally stable structure identical to that of the native exosporium. Like the exosporium, CsxA arrays require harsh “reducing” conditions for disassembly. We conclude that in vivo, CsxA self-organizes into a highly resilient, disulfide cross-linked array decorated with additional protein appendages enveloping the forespore. This pattern is remarkably similar to that in Bacillus spores, despite a lack of protein homology. In both cases, intracellular disulfide formation is favored by the high lattice symmetry. We have identified cysteine-rich proteins in many distantly related spore formers and propose that they may adopt a similar strategy for intracellular assembly of robust protective structures.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.urlhttps://msphere.asm.org/content/5/4/e00424-20.fullen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectClostridium sporogenesen_US
dc.subjectClostridium botulinumen_US
dc.subjectsporulationen_US
dc.subjectSubject Categories::C510 Applied Microbiologyen_US
dc.titleArchitecture and self-assembly of Clostridium sporogenes and Clostridium botulinum spore surfaces illustrate a general protective strategy across spore formersen_US
dc.typeArticleen_US
dc.contributor.departmentUniversity of Sheffielden_US
dc.contributor.departmentQuadram Instituteen_US
dc.identifier.journalMSPHEREen_US
dc.date.updated2020-11-23T09:41:57Z
dc.description.noteopen access article with cc licence


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Attribution-NonCommercial-NoDerivatives 4.0 International
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