• Login
    View Item 
    •   Home
    • Research from April 2016
    • Computing
    • View Item
    •   Home
    • Research from April 2016
    • Computing
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UOBREPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartment

    My Account

    LoginRegister

    About

    AboutLearning ResourcesResearch Graduate SchoolResearch InstitutesUniversity Website

    Statistics

    Display statistics

    Effects of alternating electric field on the imaging of DNA double-helix structure by atomic force microscope

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Wang, Ying
    Ma, Ke
    Wang, Jiajia
    Li, Li
    Liu, Ziyu
    Hu, Jing
    Gao, Mingyan
    Wang, Zuobin
    Issue Date
    2020-07-22
    Subjects
    single molecule imaging
    atomic force microscope
    manipulating DNA
    AC electric field
    DNA double-helix structures
    DNA stretching
    
    Metadata
    Show full item record
    Abstract
    The effects of alternating electric field on the imaging of DNA double-helix structure were explored by atomic force microscope (AFM). First, the DNA sample was located under an alternating electric field in a fixed direction and dried. Then, AFM was used to obtain the DNA images under different alternating electric fields with the voltage range from 0.5 to 6.0 V and the frequency of 50 kHz. Thus, the DNA double-helix structures with different extensions were observed when the DNA molecules were gradually stretched under different field intensities. The distributions of DNA molecules in solution were random if there were no external forces, and the curved DNA molecules were observed in the AFM image. With the increase in alternating electric voltage (0.5–4.0 V), the DNA structure was shifted from random to oriented conformation and the DNA grooves were further unfolded. While the higher voltage (5.0–6.0 V) resulted in the rupture of DNA chains due to the excessive stretching force. It showed that the optimal voltage was 1.0 V, and the double-helix structure was observed. This method provides an efficient way for monitoring and measuring bio-macromolecules. It may also enable the exploration of the DNA–protein binding and DNA molecular self-assembly processes.
    Citation
    Wang Y, Ma K, Wang J, Wang Y, Li L, Liu Z, Hu J, Gao M, Wang Z (2020) 'Effects of alternating electric field on the imaging of DNA double-helix structure by atomic force microscope', Applied Nanoscience, 10, pp.3517-3524.
    Publisher
    Springer
    Journal
    Applied Nanoscience
    URI
    http://hdl.handle.net/10547/624393
    DOI
    10.1007/s13204-020-01509-5
    Additional Links
    https://link.springer.com/article/10.1007%2Fs13204-020-01509-5
    Type
    Article
    Language
    en
    ISSN
    2190-5509
    EISSN
    2190-5517
    ae974a485f413a2113503eed53cd6c53
    10.1007/s13204-020-01509-5
    Scopus Count
    Collections
    Computing

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.