Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Name:
1-s2.0-S0925400513010514-main.pdf
Size:
1.220Mb
Format:
PDF
Description:
final version
Authors
Kang, JingHussain, Anisah T.
Catt, Michael
Trenell, Michael I.
Haggett, Barry G.D.
Yu, Eileen Hao
Issue Date
2013-09-12Subjects
non-esterified fatty acidspoly(dimethyldiallyammonium chloride)
diabetes
layer-by-layer assembly
enzyme reaction
biosensor
Metadata
Show full item recordAbstract
In this study, detection and measurement of non-esterified fatty acids (NEFA) concentration has been achieved by electrochemical method in one operation step. Multilayer films of poly(dimethyldiallyammonium chloride) (PDA) wrapped multi-wall carbon nanotubes (MWCNTs) and two enzymes acyl-CoA synthetase (ACS) and acyl-CoA oxidase (ACOD) were assembled on a carbon screen printed electrode by the layer-by-layer (LbL) immobilization. The fine polymer-enzyme layers produced by the LbL method, allowed mass transport from the reactant cascading down the layers to accomplish the two-step enzyme reactions. The polymer-CNTs and enzyme modified electrode exhibited good electrocatalytical property retaining enzyme activity. Linear increase of anodic current from H2O2 produced from NEFA oxidation was observed with the increasing concentrations of oleic acid. These results indicate a promising technique for a simple, rapid one-step determination of NEFA for diabetes management.Citation
Kang J, Hussain AT, Catt M, Trenell M, Haggett B, Yu EH (2014) 'Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes', Sensors and Actuators B: Chemical, 190, pp.535-541.Publisher
ElsevierAdditional Links
https://www.sciencedirect.com/science/article/pii/S0925400513010514Type
ArticleLanguage
enISSN
0925-4005ae974a485f413a2113503eed53cd6c53
10.1016/j.snb.2013.09.011