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    Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes

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    1-s2.0-S0925400513010514-main.pdf
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    Authors
    Kang, Jing
    Hussain, Anisah T.
    Catt, Michael
    Trenell, Michael I.
    Haggett, Barry G.D.
    Yu, Eileen Hao
    Affiliation
    Newcastle University
    University of Bedfordshire
    Issue Date
    2013-09-12
    Subjects
    non-esterified fatty acids
    poly(dimethyldiallyammonium chloride)
    diabetes
    layer-by-layer assembly
    enzyme reaction
    biosensor
    
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    Abstract
    In this study, detection and measurement of non-esterified fatty acids (NEFA) concentration has been achieved by electrochemical method in one operation step. Multilayer films of poly(dimethyldiallyammonium chloride) (PDA) wrapped multi-wall carbon nanotubes (MWCNTs) and two enzymes acyl-CoA synthetase (ACS) and acyl-CoA oxidase (ACOD) were assembled on a carbon screen printed electrode by the layer-by-layer (LbL) immobilization. The fine polymer-enzyme layers produced by the LbL method, allowed mass transport from the reactant cascading down the layers to accomplish the two-step enzyme reactions. The polymer-CNTs and enzyme modified electrode exhibited good electrocatalytical property retaining enzyme activity. Linear increase of anodic current from H2O2 produced from NEFA oxidation was observed with the increasing concentrations of oleic acid. These results indicate a promising technique for a simple, rapid one-step determination of NEFA for diabetes management.
    Citation
    Kang J, Hussain AT, Catt M, Trenell M, Haggett B, Yu EH (2014) 'Electrochemical detection of non-esterified fatty acid by layer-by-layer assembled enzyme electrodes', Sensors and Actuators B: Chemical, 190, pp.535-541.
    Publisher
    Elsevier
    Journal
    Sensors and Actuators B: Chemical
    URI
    http://hdl.handle.net/10547/624304
    DOI
    10.1016/j.snb.2013.09.011
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0925400513010514
    Type
    Article
    Language
    en
    ISSN
    0925-4005
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.snb.2013.09.011
    Scopus Count
    Collections
    Biomedical and biological science

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