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dc.contributor.authorVakati, Vinoden
dc.contributor.authorDodsworth, Stevenen
dc.date.accessioned2020-04-22T08:20:28Z
dc.date.available2020-04-22T08:20:28Z
dc.date.issued2020-01-21
dc.identifier.citationVakati V., Dodsworth S. (2020) 'Non-destructive genome skimming for aquatic copepods', Conservation Genetics Resources, (), pp.-.en
dc.identifier.issn1877-7252
dc.identifier.doi10.1007/s12686-020-01129-9
dc.identifier.urihttp://hdl.handle.net/10547/623947
dc.description.abstractCopepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttps://link.springer.com/article/10.1007/s12686-020-01129-9en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjecthigh-throughput sequencingen
dc.subjectsystematicsen
dc.subjectgenome skimmingen
dc.subjectnon-destructive DNA extractionen
dc.subjectmitogenomicsen
dc.subjectDNA barcodingen
dc.subjectC410 Applied Geneticsen
dc.titleNon-destructive genome skimming for aquatic copepodsen
dc.typeArticleen
dc.contributor.departmentNeijiang Normal Universityen
dc.contributor.departmentHanyang Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalConservation Genetics Resourcesen
dc.date.updated2020-04-22T08:17:28Z
dc.description.noteopen access article
html.description.abstractCopepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.


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