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dc.contributor.authorQu, Yingminen
dc.contributor.authorLiu, Jinyunen
dc.contributor.authorWang, Guoliangen
dc.contributor.authorSong, Zhengxunen
dc.contributor.authorWang, Zuobinen
dc.date.accessioned2020-02-28T12:12:13Z
dc.date.available2020-02-28T12:12:13Z
dc.date.issued2018-02-08
dc.identifier.citationQu Y, Liu J, Wang G, Song Z, Wang Z (2018) 'Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope', 2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Shanghai, Institute of Electrical and Electronics Engineers Inc..en
dc.identifier.isbn9781538610817
dc.identifier.doi10.1109/3M-NANO.2017.8286333
dc.identifier.urihttp://hdl.handle.net/10547/623872
dc.description.abstractIn this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.
dc.language.isoenen
dc.publisherInstitute of Electrical and Electronics Engineers Inc.en
dc.relation.urlhttps://ieeexplore.ieee.org/document/8286333en
dc.subjectatomic force microscopeen
dc.subjectTRAILen
dc.subjecthuman colon cancer cellsen
dc.titleControlled manipulation of TRAIL into single human colon cancer cells using atomic force microscopeen
dc.typeConference papers, meetings and proceedingsen
dc.contributor.departmentChangchun University of Science and Technologyen
dc.contributor.departmentUniversity of Bedfordshireen
dc.date.updated2020-02-28T12:10:06Z
html.description.abstractIn this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.


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