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    Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope

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    Authors
    Qu, Yingmin
    Liu, Jinyun
    Wang, Guoliang
    Song, Zhengxun
    Wang, Zuobin
    Affiliation
    Changchun University of Science and Technology
    University of Bedfordshire
    Issue Date
    2018-02-08
    Subjects
    atomic force microscope
    TRAIL
    human colon cancer cells
    
    Metadata
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    Abstract
    In this study, an AFM tip was used to penetrate the human colon cancer cells (SW480) in the culture medium containing pEGFP-N1-TRAIL plasmids. The trail plasmids encoded with the enhanced green fluorescent protein (EGFP) were moved into the SW480 cells through membrane holes created by the AFM probe. Following the penetration, the culture medium was changed into the RPMI1640 medium supplemented with 10% of fetal bovine serum and incubated for 24h. The expression of PEGFP-N1-TRAIL in SW480 cells was then observed by inverted fluorescence microscope. The experiment results indicate that the AFM tip can be used to penetrate the membranes of targeted cells individually.
    Citation
    Qu Y, Liu J, Wang G, Song Z, Wang Z (2018) 'Controlled manipulation of TRAIL into single human colon cancer cells using atomic force microscope', 2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Shanghai, Institute of Electrical and Electronics Engineers Inc..
    Publisher
    Institute of Electrical and Electronics Engineers Inc.
    URI
    http://hdl.handle.net/10547/623872
    DOI
    10.1109/3M-NANO.2017.8286333
    Additional Links
    https://ieeexplore.ieee.org/document/8286333
    Type
    Conference papers, meetings and proceedings
    Language
    en
    ISBN
    9781538610817
    ae974a485f413a2113503eed53cd6c53
    10.1109/3M-NANO.2017.8286333
    Scopus Count
    Collections
    Computing

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