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dc.contributor.authorWang, Xuanen
dc.contributor.authorZhao, Yujingen
dc.contributor.authorZhang, Wenxiaoen
dc.contributor.authorWang, Yingen
dc.contributor.authorTian, Liguoen
dc.contributor.authorWang, Xinyueen
dc.contributor.authorSong, Zhengxunen
dc.contributor.authorWang, Zuobinen
dc.contributor.authorLi, Dayouen
dc.date.accessioned2020-02-07T14:15:57Z
dc.date.available2020-02-07T14:15:57Z
dc.date.issued2018-11-29
dc.identifier.citationWang X, Zhao Y, Zhang W, Wang Y, Tian L, Wang X, Song Z, Wang Z, Li D (2018) 'Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope', IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Hangzhou, Institute of Electrical and Electronics Engineers Inc..en
dc.identifier.isbn9781538662144
dc.identifier.doi10.1109/3M-NANO.2018.8552210
dc.identifier.urihttp://hdl.handle.net/10547/623822
dc.description.abstractThis paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.
dc.language.isoenen
dc.publisherInstitute of Electrical and Electronics Engineers Inc.en
dc.relation.urlhttps://ieeexplore.ieee.org/document/8552210en
dc.subjectatomic force acoustic microscopeen
dc.subjectcell curingen
dc.subjectcured cell imagingen
dc.subjectA549 cellen
dc.titleDetermination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscopeen
dc.typeConference papers, meetings and proceedingsen
dc.contributor.departmentChangchun University of Science and Technologyen
dc.contributor.departmentUniversity of Bedfordshireen
dc.date.updated2020-02-07T14:07:15Z
html.description.abstractThis paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.


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