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    Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope

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    Authors
    Wang, Xuan
    Zhao, Yujing
    Zhang, Wenxiao
    Wang, Ying
    Tian, Liguo
    Wang, Xinyue
    Song, Zhengxun
    Wang, Zuobin
    Li, Dayou
    Affiliation
    Changchun University of Science and Technology
    University of Bedfordshire
    Issue Date
    2018-11-29
    Subjects
    atomic force acoustic microscope
    cell curing
    cured cell imaging
    A549 cell
    
    Metadata
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    Abstract
    This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.
    Citation
    Wang X, Zhao Y, Zhang W, Wang Y, Tian L, Wang X, Song Z, Wang Z, Li D (2018) 'Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope', IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Hangzhou, Institute of Electrical and Electronics Engineers Inc..
    Publisher
    Institute of Electrical and Electronics Engineers Inc.
    URI
    http://hdl.handle.net/10547/623822
    DOI
    10.1109/3M-NANO.2018.8552210
    Additional Links
    https://ieeexplore.ieee.org/document/8552210
    Type
    Conference papers, meetings and proceedings
    Language
    en
    ISBN
    9781538662144
    ae974a485f413a2113503eed53cd6c53
    10.1109/3M-NANO.2018.8552210
    Scopus Count
    Collections
    Computing

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