Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope
Authors
Wang, XuanZhao, Yujing
Zhang, Wenxiao
Wang, Ying
Tian, Liguo
Wang, Xinyue
Song, Zhengxun
Wang, Zuobin
Li, Dayou
Issue Date
2018-11-29
Metadata
Show full item recordAbstract
This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.Citation
Wang X, Zhao Y, Zhang W, Wang Y, Tian L, Wang X, Song Z, Wang Z, Li D (2018) 'Determination of optimal curing conditions for imaging single lung cancer cells by atomic force acoustic microscope', IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) - Hangzhou, Institute of Electrical and Electronics Engineers Inc..Additional Links
https://ieeexplore.ieee.org/document/8552210Type
Conference papers, meetings and proceedingsLanguage
enISBN
9781538662144ae974a485f413a2113503eed53cd6c53
10.1109/3M-NANO.2018.8552210