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dc.contributor.authorHu, Jingen
dc.contributor.authorGao, Mingyanen
dc.contributor.authorWang, Yingen
dc.contributor.authorLiu, Mengnanen
dc.contributor.authorWang, Jianfeien
dc.contributor.authorLi, Jianien
dc.contributor.authorSong, Zhengxunen
dc.contributor.authorChen, Yujuanen
dc.contributor.authorWang, Zuobinen
dc.date.accessioned2020-01-28T12:36:40Z
dc.date.available2020-01-28T12:36:40Z
dc.date.issued2019-10-29
dc.identifier.citationHu J, Gao M, Wang Y, Liu M, Wang J, Li J, Song Z, Chen Y, Wang Z (2019) 'Imaging the substructures of individual IgE antibodies with atomic force microscopy', Langmuir, 35 (46), pp.14896-14901.en
dc.identifier.issn0743-7463
dc.identifier.doi10.1021/acs.langmuir.9b02631
dc.identifier.urihttp://hdl.handle.net/10547/623801
dc.description.abstractThe interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly.
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.urlhttps://pubs.acs.org/doi/abs/10.1021/acs.langmuir.9b02631en
dc.rightsWhite - archiving not formally supported
dc.subjectatomic force microscopyen
dc.titleImaging the substructures of individual IgE antibodies with atomic force microscopyen
dc.typeArticleen
dc.identifier.journalLangmuiren
dc.date.updated2020-01-28T12:29:03Z
dc.description.noteUnfortunately the publisher will not allow us to archive the final pdf - do you have a previous version we can use? E.g. the version after review, but before the publisher formatting applied. over 3 months from publication and no response from researcher, passing metadata only RVO 28/1/20
html.description.abstractThe interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly.


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