Imaging the substructures of individual IgE antibodies with atomic force microscopy
dc.contributor.author | Hu, Jing | en |
dc.contributor.author | Gao, Mingyan | en |
dc.contributor.author | Wang, Ying | en |
dc.contributor.author | Liu, Mengnan | en |
dc.contributor.author | Wang, Jianfei | en |
dc.contributor.author | Li, Jiani | en |
dc.contributor.author | Song, Zhengxun | en |
dc.contributor.author | Chen, Yujuan | en |
dc.contributor.author | Wang, Zuobin | en |
dc.date.accessioned | 2020-01-28T12:36:40Z | |
dc.date.available | 2020-01-28T12:36:40Z | |
dc.date.issued | 2019-10-29 | |
dc.identifier.citation | Hu J, Gao M, Wang Y, Liu M, Wang J, Li J, Song Z, Chen Y, Wang Z (2019) 'Imaging the substructures of individual IgE antibodies with atomic force microscopy', Langmuir, 35 (46), pp.14896-14901. | en |
dc.identifier.issn | 0743-7463 | |
dc.identifier.doi | 10.1021/acs.langmuir.9b02631 | |
dc.identifier.uri | http://hdl.handle.net/10547/623801 | |
dc.description.abstract | The interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly. | |
dc.language.iso | en | en |
dc.publisher | American Chemical Society | en |
dc.relation.url | https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.9b02631 | en |
dc.rights | White - archiving not formally supported | |
dc.subject | atomic force microscopy | en |
dc.title | Imaging the substructures of individual IgE antibodies with atomic force microscopy | en |
dc.type | Article | en |
dc.identifier.journal | Langmuir | en |
dc.date.updated | 2020-01-28T12:29:03Z | |
dc.description.note | Unfortunately the publisher will not allow us to archive the final pdf - do you have a previous version we can use? E.g. the version after review, but before the publisher formatting applied. over 3 months from publication and no response from researcher, passing metadata only RVO 28/1/20 | |
html.description.abstract | The interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly. |