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    Imaging the substructures of individual IgE antibodies with atomic force microscopy

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    Authors
    Hu, Jing
    Gao, Mingyan
    Wang, Ying
    Liu, Mengnan
    Wang, Jianfei
    Li, Jiani
    Song, Zhengxun
    Chen, Yujuan
    Wang, Zuobin
    Issue Date
    2019-10-29
    Subjects
    atomic force microscopy
    
    Metadata
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    Abstract
    The interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly.
    Citation
    Hu J, Gao M, Wang Y, Liu M, Wang J, Li J, Song Z, Chen Y, Wang Z (2019) 'Imaging the substructures of individual IgE antibodies with atomic force microscopy', Langmuir, 35 (46), pp.14896-14901.
    Publisher
    American Chemical Society
    Journal
    Langmuir
    URI
    http://hdl.handle.net/10547/623801
    DOI
    10.1021/acs.langmuir.9b02631
    Additional Links
    https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.9b02631
    Type
    Article
    Language
    en
    ISSN
    0743-7463
    ae974a485f413a2113503eed53cd6c53
    10.1021/acs.langmuir.9b02631
    Scopus Count
    Collections
    Computing

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