Imaging the substructures of individual IgE antibodies with atomic force microscopy
Authors
Hu, JingGao, Mingyan
Wang, Ying
Liu, Mengnan
Wang, Jianfei
Li, Jiani
Song, Zhengxun
Chen, Yujuan
Wang, Zuobin
Issue Date
2019-10-29Subjects
atomic force microscopy
Metadata
Show full item recordAbstract
The interaction between antibodies and substrates directly affects its conformation and thus its immune function. Therefore, it is desirable to study the structure of antibodies at the single molecule level. Herein, the substructures of Immunoglobulin E (IgE) on solid surfaces were investigated. For this purpose, the tapping-mode atomic force microscopy (AFM) was applied to observe the individual IgE substructures adsorbed onto Mg2+ and Na+ modified mica substrates in air. As expected, the AFM images revealed that the IgE antibodies exhibited different conformations on the surface of mica substrate, consisting of the four basic orientations: three domain, two equivalent domain, two unequal domain and single domain morphologies. Moreover, the differences of the different orientations in single IgE antibodies were also identified clearly.Citation
Hu J, Gao M, Wang Y, Liu M, Wang J, Li J, Song Z, Chen Y, Wang Z (2019) 'Imaging the substructures of individual IgE antibodies with atomic force microscopy', Langmuir, 35 (46), pp.14896-14901.Publisher
American Chemical SocietyJournal
LangmuirAdditional Links
https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.9b02631Type
ArticleLanguage
enISSN
0743-7463ae974a485f413a2113503eed53cd6c53
10.1021/acs.langmuir.9b02631