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    Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase

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    Authors
    Liu, Ziya
    Xu, Hongmei
    Wang, Ying
    Yang, Fan
    Yin, Yaoting
    Zhang, Sheng
    Weng, Zhankun
    Song, Zhengxun
    Wang, Zuobin
    Affiliation
    Changchun University of Science and Technology
    University of Bedfordshire
    Issue Date
    2019-05-25
    Subjects
    ion modification
    electric field
    AFM imaging
    DNA straightening
    liquid phase
    C440 Molecular Genetics
    
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    Abstract
    In this research, a novel method is proposed to improve DNA straightening under an applied electric field to facilitate imaging in a liquid phase by modifying the substrate with varying Mg2+ ion concentrations. A two-dimensional network of DNA structures was successfully stretched on Mg2+-modified mica substrates under a DC electric field (1 V, 1 A) and imaged in gaseous and aqueous phases by atomic force microscopy. The results revealed that an optimum concentration of Mg2+ ion (4.17 μmol/ml) allowed DNA straightening under an electric field, thus facilitating its imaging in the liquid phase. Furthermore, DNA adhesion under different concentrations of Mg2+ was measured and a maximum adhesion force of 76.19 pN was achieved. This vital work has great potential in gene knockout and targeted gene editing.
    Citation
    Liu Z, Xu H, Wang Y, Yang F, Yin Y, Zhang S, Weng Z, Song Z, Wang Z (2019) 'Improved DNA straightening and attachment via optimal Mg2+ ionic bonding under electric field for AFM imaging in liquid phase', Micron, 124 (), pp.102678-.
    Publisher
    Elsevier Ltd
    Journal
    Micron
    URI
    http://hdl.handle.net/10547/623755
    DOI
    10.1016/j.micron.2019.04.006
    PubMed ID
    31181466
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S096843281930068X
    Type
    Article
    Language
    en
    ISSN
    0968-4328
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.micron.2019.04.006
    Scopus Count
    Collections
    Computing

    entitlement

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