Analysis of colchicine-induced effects on hepatoma and hepatpcyte cells by atomic force microscopy
Issue Date
2018-06-01Subjects
atomic force microscopebiomechanical properties
colchicine
hepatocyte (HL-7702)
hepatoma cell (SMCC-7721)
B830 Biomechanics, Biomaterials and Prosthetics (non-clinical)
Metadata
Show full item recordAbstract
Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale. The cells were exposed to a solution with a normal dose of colchicine for two, four and six hours. Surface topographic images showed that colchicine decreased the stability of the cytoskeleton. After the same six-hour treatment in a solution with a normal dose of colchicine, the biomechanical properties of HL-7702 cells were almost unchanged. However, the stiffness and the adhesion force of the SMCC-7721 cells were clearly increased (more than twofold of the normal values), especially after four hours. The deformability of SMCC-7721 cancer cells was significantly decreased within the six-hour treatment in the solution with a normal dose of colchicine. Analysis of the biomechanical properties of post-treatment hepatoma cells provided a complementary explanation for the mechanism of action of colchicine on cells at the nanoscale. This method is expected to allow the monitoring of potential metastatic cancer cell changes, thus preventing the emergence and the transmission of disease, and improving the diagnosis of cancer.Citation
Liu L, Wang Z, Zhang W, Zhu X, Li L, Weng Z (2018) 'Analysis of colchicine-induced effects on hepatoma and hepatpcyte cells by atomic force microscopy', Journal of Nanoscience and Nanotechnology, 18 (6), pp.4248-4254.Publisher
American Scientific PublishersPubMed ID
29442770Additional Links
https://doi.org/10.1166/jnn.2018.15193Type
ArticleLanguage
enISSN
1533-4880ae974a485f413a2113503eed53cd6c53
10.1166/jnn.2018.15193