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    Analysis of colchicine-induced effects on hepatoma and hepatpcyte cells by atomic force microscopy

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    Authors
    Liu, Lanjiao
    Wang, Zuobin
    Zhang, Wenxiao
    Zhu, Xinyao
    Li, Li
    Weng, Zhankun
    Affiliation
    Changchun University of Science and Technology
    University of Warwick
    Issue Date
    2018-06-01
    Subjects
    atomic force microscope
    biomechanical properties
    colchicine
    hepatocyte (HL-7702)
    hepatoma cell (SMCC-7721)
    B830 Biomechanics, Biomaterials and Prosthetics (non-clinical)
    
    Metadata
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    Abstract
    Biomechanical properties of cells are altered by many diseases. Cancer cell metastasis is related to the properties such as the cell stiffness that influences cell proliferation, differentiation and migration. In this paper, we used an atomic force microscope to analyze the colchicine-induced effects on the mechanical properties of hepatocyte (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture at the nanoscale. The cells were exposed to a solution with a normal dose of colchicine for two, four and six hours. Surface topographic images showed that colchicine decreased the stability of the cytoskeleton. After the same six-hour treatment in a solution with a normal dose of colchicine, the biomechanical properties of HL-7702 cells were almost unchanged. However, the stiffness and the adhesion force of the SMCC-7721 cells were clearly increased (more than twofold of the normal values), especially after four hours. The deformability of SMCC-7721 cancer cells was significantly decreased within the six-hour treatment in the solution with a normal dose of colchicine. Analysis of the biomechanical properties of post-treatment hepatoma cells provided a complementary explanation for the mechanism of action of colchicine on cells at the nanoscale. This method is expected to allow the monitoring of potential metastatic cancer cell changes, thus preventing the emergence and the transmission of disease, and improving the diagnosis of cancer.
    Citation
    Liu L, Wang Z, Zhang W, Zhu X, Li L, Weng Z (2018) 'Analysis of colchicine-induced effects on hepatoma and hepatpcyte cells by atomic force microscopy', Journal of Nanoscience and Nanotechnology, 18 (6), pp.4248-4254.
    Publisher
    American Scientific Publishers
    Journal
    Journal of Nanoscience and Nanotechnology
    URI
    http://hdl.handle.net/10547/623568
    DOI
    10.1166/jnn.2018.15193
    PubMed ID
    29442770
    Additional Links
    https://doi.org/10.1166/jnn.2018.15193
    Type
    Article
    Language
    en
    ISSN
    1533-4880
    ae974a485f413a2113503eed53cd6c53
    10.1166/jnn.2018.15193
    Scopus Count
    Collections
    Computing

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