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dc.contributor.authorQu, Yingminen
dc.contributor.authorWang, Zuobinen
dc.contributor.authorZhao, Feihuen
dc.contributor.authorLiu, Jinyunen
dc.contributor.authorZhang, Wenxiaoen
dc.contributor.authorLi, Jingmeien
dc.contributor.authorSong, Zhengxunen
dc.contributor.authorXu, Hongmeien
dc.date.accessioned2019-10-31T10:29:06Z
dc.date.available2019-10-31T10:29:06Z
dc.date.issued2017-10-12
dc.identifier.citationQu Y, Wang Z, Zhao F, Liu J, Zhang W, Li J, Song Z, Xu H (2018) 'AFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extract', Micron, 104, pp.1-7.en
dc.identifier.issn0968-4328
dc.identifier.pmid29049926
dc.identifier.doi10.1016/j.micron.2017.10.003
dc.identifier.urihttp://hdl.handle.net/10547/623559
dc.description.abstractAmerican ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE). The results showed that the cells treated with AGRWE exhibited significantly larger surface roughness, height and elastic modulus values than control group. Moreover, those parameters were upregulated under the higher concentration of AGRWE and longer culture time. Consequently, it indicates that the mechanical and morphological properties can be used as the apoptotic characteristics of SMMC-7721 cells. Also, the increased surface roughness and elastic modulus of cells under the AGRWE treatment have shown that the apoptosis of SMMC-7721 cells can be enhanced by AGRWE. This will provide an important implication for hepatocelluar carcinoma treatment and drug development.
dc.description.sponsorship“111” Project of China (D17017), EU FP7 (BioRA No.612641), China-EU H2020 (FabSurfWAR Nos.2016YFE0112100 and 644971), EU H2020 (MNR4SCell No.734174), and Jilin Provincial Science and Technology Program (Nos.20160520101JH, 20160101318JC and 20160623002TC)en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0968432817303256en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.subjectginseng-extracted compoundsen
dc.subjectAmerican ginsengen
dc.subjectatomic force microscopeen
dc.subjectatomic force microscopyen
dc.subjectcell apoptosisen
dc.subjectcellular stiffnessen
dc.subjectSMMC-7721 cellsen
dc.subjecthepatocelluar carcinomaen
dc.subjectC130 Cell Biologyen
dc.titleAFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extracten
dc.typeArticleen
dc.contributor.departmentChangchun University of Science and Technologyen
dc.contributor.departmentEindhoven University of Technologyen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalMicronen
dc.date.updated2019-10-31T09:58:45Z
dc.description.notenot chasing for full text as beyond 3 months from publication
html.description.abstractAmerican ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE). The results showed that the cells treated with AGRWE exhibited significantly larger surface roughness, height and elastic modulus values than control group. Moreover, those parameters were upregulated under the higher concentration of AGRWE and longer culture time. Consequently, it indicates that the mechanical and morphological properties can be used as the apoptotic characteristics of SMMC-7721 cells. Also, the increased surface roughness and elastic modulus of cells under the AGRWE treatment have shown that the apoptosis of SMMC-7721 cells can be enhanced by AGRWE. This will provide an important implication for hepatocelluar carcinoma treatment and drug development.


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