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dc.contributor.authorLiu, Lanjiaoen
dc.contributor.authorZhang, Wenxiaoen
dc.contributor.authorLi, Lien
dc.contributor.authorZhu, Xinyaoen
dc.contributor.authorLiu, Jinyunen
dc.contributor.authorWang, Xinyueen
dc.contributor.authorSong, Zhengxunen
dc.contributor.authorXu, Hongmeien
dc.contributor.authorWang, Zuobinen
dc.date.accessioned2019-10-31T10:28:44Z
dc.date.available2019-10-31T10:28:44Z
dc.date.issued2017-12-17
dc.identifier.citationLiu L, Zhang W, L Li, Zhu X, Liu J, Wang X, Song Z, Xu H, Wang Z (2018) 'Biomechanical measurement and analysis of colchicine-induced effects on cells by nanoindentation using an atomic force microscope', Journal of Biomechanics, 76 (23), pp.84-90.en
dc.identifier.issn0021-9290
dc.identifier.pmid29249455
dc.identifier.doi10.1016/j.jbiomech.2017.11.018
dc.identifier.urihttp://hdl.handle.net/10547/623555
dc.description.abstractColchicine is a drug commonly used for the treatment of gout, however, patients may sometimes encounter side-effects induced by taking colchicine, such as nausea, vomiting, diarrhea and kidney failure. In this regard, it is imperative to investigate the mechanism effects of colchicine on biological cells. In this paper, we present a method for the detection of mechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture by atomic force microscope(AFM) to analyze the 0.1 μg/mL colchicine-induced effects on the nanoscalefor two, four and six hours. Compared to the corresponding control cells, the biomechanical properties of the VERO and SMCC-7721 cells changed significantly and the HL-7702 cells did not considerably change after the treatment when considering the same time period. Based on biomechanical property analyses, the colchicine solution made the VERO and SMCC-7721 cells harder. We conclude that it is possible to reduce the division rate of the VERO cells and inhibit the metastasis of the SMCC-7721 cells. The method described here can be applied to study biomechanics of many other types of cells with different drugs. Therefore, this work provides an accurate and rapid method for drug screening and mechanical analysis of cells in medical research.
dc.description.sponsorshipEU FP7 (BioRA No. 612641), China-EU H2020 (FabSurfWAR Nos. 2016YFE0112100 and 644971), EU H2020 (MNR4SCell No. 734174), and Jilin Provincial Science and Technology Program (Nos. 20140414009GH, 20140622009JC and 20160623002TC). These sponsors had no role in the study design or the writing of the manuscript, or the decision to submit the manuscript for publication.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0021929017306693en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectatomic force microscopeen
dc.subjectcolchicineen
dc.subjectmechanical propertiesen
dc.subjectliving cellsen
dc.subjectatomic force microscopyen
dc.subjectC130 Cell Biologyen
dc.titleBiomechanical measurement and analysis of colchicine-induced effects on cells by nanoindentation using an atomic force microscopeen
dc.typeArticleen
dc.contributor.departmentChangchun University of Science and Technologyen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalJournal of Biomechanicsen
dc.date.updated2019-10-31T09:58:42Z
refterms.dateFOA2020-04-23T08:42:04Z
html.description.abstractColchicine is a drug commonly used for the treatment of gout, however, patients may sometimes encounter side-effects induced by taking colchicine, such as nausea, vomiting, diarrhea and kidney failure. In this regard, it is imperative to investigate the mechanism effects of colchicine on biological cells. In this paper, we present a method for the detection of mechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture by atomic force microscope(AFM) to analyze the 0.1 μg/mL colchicine-induced effects on the nanoscalefor two, four and six hours. Compared to the corresponding control cells, the biomechanical properties of the VERO and SMCC-7721 cells changed significantly and the HL-7702 cells did not considerably change after the treatment when considering the same time period. Based on biomechanical property analyses, the colchicine solution made the VERO and SMCC-7721 cells harder. We conclude that it is possible to reduce the division rate of the VERO cells and inhibit the metastasis of the SMCC-7721 cells. The method described here can be applied to study biomechanics of many other types of cells with different drugs. Therefore, this work provides an accurate and rapid method for drug screening and mechanical analysis of cells in medical research.


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