• Login
    View Item 
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UOBREPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartment

    My Account

    LoginRegister

    About

    AboutLearning ResourcesResearch Graduate SchoolResearch InstitutesUniversity Website

    Statistics

    Display statistics

    Intramolecular polyspecificity in CD4 T-cell recognition of Ad-restricted epitopes of proteoglycan aggrecan

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Falconer, Jane
    Lowes, Katie
    Furmanski, Anna L.
    Dyson, Julian
    Ng, Wan Fai
    Robinson, John H.
    Issue Date
    2013-12-24
    Subjects
    T cell
    C550 Immunology
    
    Metadata
    Show full item record
    Abstract
    T-cell recognition of MHC–peptide complexes shows a high degree of polyspecificity extending to recognition of a large number of structurally unrelated peptides. Examples of polyspecificity reported to date are confined to recognition of epitopes from distinct proteins or synthetic peptide libraries. Here we describe intramolecular polyspecificity of CD4 T cells specific for several epitopes within proteoglycan aggrecan, a structural glycoprotein of cartilage and candidate autoantigen in rheumatoid arthritis. T-cell hybridomas from aggrecan-immunized mice recognized four structurally unrelated epitopes from the G1 domain of aggrecan, but not other aggrecan epitopes or a variety of other peptide epitopes restricted by the same MHC class II allele. We also showed that the hierarchy of cross-reactivity broadly correlated with the strength of peptide binding to MHC class II. Similar polyspecificity was observed in responses of lymph node cells from peptide-immunized mice, suggesting polyspecificity of a significant proportion of the in vivo aggrecan specific T-cell repertoire. Polyspecific recognition of several epitopes within the same autoantigen may provide a novel mechanism to reach the activation threshold of low-affinity autoreactive T cells in the initiation of autoimmune diseases.
    Citation
    Falconer J, Lowes K, Furmanski AL, Dyson J, Ng WF, Robinson JH (2014) 'Intramolecular polyspecificity in CD4 T-cell recognition of Ad-restricted epitopes of proteoglycan aggrecan', Immunology, 142 (1), pp.101-110.
    Publisher
    Wiley
    Journal
    Immunology
    URI
    http://hdl.handle.net/10547/623403
    DOI
    10.1111/imm.12238
    PubMed ID
    24843873
    PubMed Central ID
    PMC3992052
    Additional Links
    https://onlinelibrary.wiley.com/doi/full/10.1111/imm.12238
    Type
    Article
    Language
    en
    ISSN
    0019-2805
    ae974a485f413a2113503eed53cd6c53
    10.1111/imm.12238
    Scopus Count
    Collections
    Biomedical and biological science

    entitlement

    Related articles

    • Unconventional T-cell recognition of an arthritogenic epitope of proteoglycan aggrecan released from degrading cartilage.
    • Authors: Falconer J, Mahida R, Venkatesh D, Pearson J, Robinson JH
    • Issue date: 2016 Apr
    • Induction of arthritis in SCID mice by T cells specific for the "shared epitope" sequence in the G3 domain of human cartilage proteoglycan.
    • Authors: Hanyecz A, Bárdos T, Berlo SE, Buzás E, Nesterovitch AB, Mikecz K, Glant TT
    • Issue date: 2003 Oct
    • The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis.
    • Authors: Misják P, Bősze S, Horváti K, Pásztói M, Pálóczi K, Holub MC, Szakács F, Aradi B, György B, Szabó TG, Nagy G, Glant TT, Mikecz K, Falus A, Buzás EI
    • Issue date: 2013 Apr
    • Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis.
    • Authors: Zou J, Zhang Y, Thiel A, Rudwaleit M, Shi SL, Radbruch A, Poole R, Braun J, Sieper J
    • Issue date: 2003 Jul
    • Arthritis induced by proteoglycan aggrecan G1 domain in BALB/c mice. Evidence for t cell involvement and the immunosuppressive influence of keratan sulfate on recognition of t and b cell epitopes.
    • Authors: Zhang Y, Guerassimov A, Leroux JY, Cartman A, Webber C, Lalic R, de Miguel E, Rosenberg LC, Poole AR
    • Issue date: 1998 Apr 15
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.