A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles
dc.contributor.author | Zariwala, Mohammed Gulrez | en |
dc.contributor.author | Elsaid, Naba | en |
dc.contributor.author | Jackson, Timothy L. | en |
dc.contributor.author | Corral López, Francisco | en |
dc.contributor.author | Farnaud, Sébastien | en |
dc.contributor.author | Somavarapu, Satyanarayana | en |
dc.contributor.author | Renshaw, Derek | en |
dc.date.accessioned | 2019-02-15T13:45:16Z | |
dc.date.available | 2019-02-15T13:45:16Z | |
dc.date.issued | 2013-11-18 | |
dc.identifier.citation | Zariwala MG, Elsaid N, Jackson TL, Corral López F, Farnaud S, Somavarapu S, Renshaw D (2013) 'A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles', International Journal of Pharmaceutics, 456 (2), pp.400-7. | en |
dc.identifier.issn | 0378-5173 | |
dc.identifier.pmid | 24012860 | |
dc.identifier.doi | 10.1016/j.ijpharm.2013.08.070 | |
dc.identifier.uri | http://hdl.handle.net/10547/623165 | |
dc.description.abstract | Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery. | |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.url | https://www.sciencedirect.com/science/article/pii/S037851731300803X | en |
dc.rights | Green - can archive pre-print and post-print or publisher's version/PDF | |
dc.subject | iron | en |
dc.title | A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles | en |
dc.type | Article | en |
dc.identifier.eissn | 0378-5173 | |
dc.contributor.department | University of Westminster | en |
dc.contributor.department | University College London | en |
dc.contributor.department | King's College Hospital | en |
dc.contributor.department | University of Bedfordshire | en |
dc.identifier.journal | International Journal of Pharmaceutics | en |
dc.date.updated | 2019-02-15T13:42:41Z | |
html.description.abstract | Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery. |