A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles
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Authors
Zariwala, Mohammed GulrezElsaid, Naba
Jackson, Timothy L.
Corral López, Francisco
Farnaud, Sébastien
Somavarapu, Satyanarayana
Renshaw, Derek
Affiliation
University of WestminsterUniversity College London
King's College Hospital
University of Bedfordshire
Issue Date
2013-11-18Subjects
iron
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Iron (Fe) loaded solid lipid nanoparticles (SLN's) were formulated using stearic acid and iron absorption was evaluated in vitro using the cell line Caco-2 with intracellular ferritin formation as a marker of iron absorption. Iron loading was optimised at 1% Fe (w/w) lipid since an inverse relation was observed between initial iron concentration and SLN iron incorporation efficiency. Chitosan (Chi) was included to prepare chitosan coated SLN's. Particle size analysis revealed a sub-micron size range (300.3±31.75 nm to 495.1±80.42 nm), with chitosan containing particles having the largest dimensions. As expected, chitosan (0.1%, 0.2% and 0.4% w/v) conferred a net positive charge on the particle surface in a concentration dependent manner. For iron absorption experiments equal doses of Fe (20 μM) from selected formulations (SLN-FeA and SLN-Fe-ChiB) were added to Caco-2 cells and intracellular ferritin protein concentrations determined. Caco-2 iron absorption from SLN-FeA (583.98±40.83 ng/mg cell protein) and chitosan containing SLN-Fe-ChiB (642.77±29.37 ng/mg cell protein) were 13.42% and 24.9% greater than that from ferrous sulphate (FeSO4) reference (514.66±20.43 ng/mg cell protein) (p≤0.05). We demonstrate for the first time preparation, characterisation and superior iron absorption in vitro from SLN's, suggesting the potential of these formulations as a novel system for oral iron delivery.Citation
Zariwala MG, Elsaid N, Jackson TL, Corral López F, Farnaud S, Somavarapu S, Renshaw D (2013) 'A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles', International Journal of Pharmaceutics, 456 (2), pp.400-7.Publisher
ElsevierPubMed ID
24012860Additional Links
https://www.sciencedirect.com/science/article/pii/S037851731300803XType
ArticleLanguage
enISSN
0378-5173EISSN
0378-5173ae974a485f413a2113503eed53cd6c53
10.1016/j.ijpharm.2013.08.070
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