Ascorbyl palmitate/DSPE-PEG nanocarriers for oral iron delivery: preparation, characterisation and in vitro evaluation
dc.contributor.author | Zariwala, Mohammed Gulrez | en |
dc.contributor.author | Farnaud, Sébastien | en |
dc.contributor.author | Merchant, Zahra | en |
dc.contributor.author | Somavarapu, Satyanarayana | en |
dc.contributor.author | Renshaw, Derek | en |
dc.date.accessioned | 2019-02-15T13:39:05Z | |
dc.date.available | 2019-02-15T13:39:05Z | |
dc.date.issued | 2014-03-01 | |
dc.identifier.citation | Zariwala MG, Farnaud S, Merchant Z, Somavarapu S, Renshaw D (2014) 'Ascorbyl palmitate/DSPE-PEG nanocarriers for oral iron delivery: preparation, characterisation and in vitro evaluation', Colloids and Surfaces B: Biointerfaces, 115 (), pp.86-92. | en |
dc.identifier.issn | 0927-7765 | |
dc.identifier.pmid | 24333557 | |
dc.identifier.doi | 10.1016/j.colsurfb.2013.11.028 | |
dc.identifier.uri | http://hdl.handle.net/10547/623164 | |
dc.description.abstract | The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption. | |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.url | https://www.sciencedirect.com/science/article/pii/S0927776513007261 | en |
dc.rights | Green - can archive pre-print and post-print or publisher's version/PDF | |
dc.subject | iron | en |
dc.title | Ascorbyl palmitate/DSPE-PEG nanocarriers for oral iron delivery: preparation, characterisation and in vitro evaluation | en |
dc.type | Article | en |
dc.identifier.eissn | 0927-7765 | |
dc.contributor.department | University of Westminster | en |
dc.contributor.department | University of Bedfordshire | en |
dc.contributor.department | UCL School of Pharmacy | en |
dc.identifier.journal | Colloids and Surfaces B: Biointerfaces | en |
dc.date.updated | 2019-02-15T13:36:24Z | |
html.description.abstract | The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption. |