• Login
    View Item 
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UOBREPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartment

    My Account

    LoginRegister

    About

    AboutLearning ResourcesResearch Graduate SchoolResearch InstitutesUniversity Website

    Statistics

    Display statistics

    Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Authors
    Mehta, Kosha
    Greenwell, Pamela
    Renshaw, Derek
    Busbridge, Mark
    Garcia, Mitla
    Farnaud, Sébastien
    Patel, Vinood B.
    Affiliation
    University of Westminster
    Coventry University
    Imperial College Healthcare NHS Trust
    King's College London
    University of Bedfordshire
    Issue Date
    2015-08-28
    Subjects
    hepcidin
    
    Metadata
    Show full item record
    Abstract
    Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO TRVb-1) cells were used that express iron-response-element-depleted human TFRC mRNA (TFRC∆IRE). Results showed that CHO TRVb-1 cells expressed higher basal levels of cell-surface TFR1 than HepG2 cells (2.2-fold; p < 0.01) and following 5 g/L holotransferrin treatment maintained constitutive over-expression at 24h and 48 h, contrasting the HepG2 cells where the receptor levels significantly declined. Despite this, the intracellular iron content was neither higher than HepG2 cells nor increased over time under basal or holotransferrin-treated conditions. Interestingly, hepcidin secretion in CHO TRVb-1 cells exceeded basal levels at all time-points (p < 0.02) and matched levels in HepG2 cells following treatment. While TFRC mRNA expression showed expected elevation (2h, p < 0.03; 4h; p < 0.05), slc40a1 mRNA expression was also elevated (2 h, p < 0.05; 4 h, p < 0.03), unlike the HepG2 cells. In conclusion, the CHO TRVb-1 cells prevented cellular iron-overload by elevating slc40a1 expression, thereby highlighting its significance in the absence of iron-regulated TFRC mRNA. Furthermore, hepcidin response to holotransferrin treatment was similar to HepG2 cells and resembled the human physiological response.
    Citation
    Mehta K, Greenwell P, Renshaw D, Busbridge M, Garcia M, Farnaud S, Patel VB (2015) 'Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells', Blood Cells, Molecules and Diseases, 55 (2), pp.110-8.
    Publisher
    Elsevier
    Journal
    Blood Cells, Molecules and Diseases
    URI
    http://hdl.handle.net/10547/623163
    DOI
    10.1016/j.bcmd.2015.05.002
    PubMed ID
    26142326
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S1079979615000807
    Type
    Article
    Language
    en
    ISSN
    1079-9796
    EISSN
    1079-9796
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bcmd.2015.05.002
    Scopus Count
    Collections
    Biomedical and biological science

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.