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dc.contributor.authorDodsworth, Stevenen
dc.contributor.authorGuignard, Maite S.en
dc.contributor.authorChristenhusz, Maarten J.M.en
dc.contributor.authorCowan, Robyn S.en
dc.contributor.authorKnapp, Sandraen
dc.contributor.authorMaurin, Olivieren
dc.contributor.authorStruebig, Monikaen
dc.contributor.authorLeitch, Andrew R.en
dc.contributor.authorChase, Mark W.en
dc.contributor.authorForest, Felixen
dc.date.accessioned2019-02-11T13:14:02Z
dc.date.available2019-02-11T13:14:02Z
dc.date.issued2018-10-29
dc.identifier.citationDodsworth S., Guignard M., Christenhusz M., Cowan R., Knapp S., Maurin O., Struebig M., Leitch A., Chase M., Forest F. (2018) 'Potential of herbariomics for studying repetitive DNA in angiosperms', Frontiers in Ecology and Evolution, 6 (), pp.-.en
dc.identifier.issn2296-701X
dc.identifier.doi10.3389/fevo.2018.00174
dc.identifier.urihttp://hdl.handle.net/10547/623134
dc.description.abstractRepetitive DNA has an important role in angiosperm genomes and is relevant to our understanding of genome size variation, polyploidisation and genome dynamics more broadly. Much recent work has harnessed the power of high-throughput sequencing (HTS) technologies to advance the study of repetitive DNA in flowering plants. Herbarium collections provide a useful historical perspective on genome diversity through time, but their value for the study of repetitive DNA has not yet been explored. We propose that herbarium DNA may prove as useful for studies of repetitive DNA content as it has for reconstructed organellar genomes and low-copy nuclear sequence data. Here we present a case study in the tobacco genus (Nicotiana; Solanaceae), showing that herbarium specimens can provide accurate estimates of the repetitive content of angiosperm genomes by direct comparison with recently-collected material. We show a strong correlation between the abundance of repeat clusters, e.g., different types of transposable elements and satellite DNA, in herbarium collections versus recent material for four sets of Nicotiana taxa. These results suggest that herbarium specimen genome sequencing (herbariomics) holds promise for both repeat discovery and analyses that aim to investigate the role of repetitive DNAs in genomic evolution, particularly genome size evolution and/or contributions of repeats to the regulation of gene space.
dc.description.sponsorshipThis work was funded by a NERC studentship to SD.en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fevo.2018.00174/fullen
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSolanaceaeen
dc.subjectherbarium specimensen
dc.subjectherbariomicsen
dc.subjectangiospermsen
dc.subjecthigh-throughput sequencingen
dc.subjectgenomicsen
dc.subjectrepetitive DNAen
dc.subjectC400 Geneticsen
dc.titlePotential of herbariomics for studying repetitive DNA in angiospermsen
dc.typeArticleen
dc.identifier.eissn2296-701X
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentQueen Mary University of Londonen
dc.contributor.departmentRoyal Botanic Gardens, Kewen
dc.contributor.departmentPlant Gateway, Bradforden
dc.contributor.departmentNatural History Museum, Londonen
dc.contributor.departmentUniversity of Western Australiaen
dc.identifier.journalFrontiers in Ecology and Evolutionen
dc.date.updated2019-02-11T12:04:35Z
dc.description.noteOA article Creative Commons Attribution License (CC BY).
html.description.abstractRepetitive DNA has an important role in angiosperm genomes and is relevant to our understanding of genome size variation, polyploidisation and genome dynamics more broadly. Much recent work has harnessed the power of high-throughput sequencing (HTS) technologies to advance the study of repetitive DNA in flowering plants. Herbarium collections provide a useful historical perspective on genome diversity through time, but their value for the study of repetitive DNA has not yet been explored. We propose that herbarium DNA may prove as useful for studies of repetitive DNA content as it has for reconstructed organellar genomes and low-copy nuclear sequence data. Here we present a case study in the tobacco genus (Nicotiana; Solanaceae), showing that herbarium specimens can provide accurate estimates of the repetitive content of angiosperm genomes by direct comparison with recently-collected material. We show a strong correlation between the abundance of repeat clusters, e.g., different types of transposable elements and satellite DNA, in herbarium collections versus recent material for four sets of Nicotiana taxa. These results suggest that herbarium specimen genome sequencing (herbariomics) holds promise for both repeat discovery and analyses that aim to investigate the role of repetitive DNAs in genomic evolution, particularly genome size evolution and/or contributions of repeats to the regulation of gene space.


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