ATP-binding cassette transporter VcaM from Vibrio cholerae is dependent on the outer membrane factor family for Its function
Authors
Lu, Wen-JungLin, Hsuan-Ju
Janganan, Thamarai K.
Li, Cheng-Yu
Chin, Wen-Chiang
Bavro, Vassiliy N
Lin, Hong-Ting Victor
Issue Date
2018-03-27
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strains lacking the principal secondary transporter AcrB (Acriflavine resistance protein), as well as cells lacking the outer membrane factor (OMF) TolC (Tolerance to colicins). Consistent with the ATPase function of VcaM we found it to be susceptible to sodium orthovanadate (NaOV), however, we also found a clear dependency of VcaM function on TolC. Inhibitors targeting secondary active transporters had no effects on either VcaM-conferred resistance or Hoechst 33342 accumulation, suggesting that VcaM might be capable of engaging with the TolC-channel without periplasmic mediation by additional transporters. Our findings are indicative of VcaM being capable of a one-step substrate translocation from cytosol to extracellular space utilising the TolC-channel, making it the only multidrug ABC-transporter outside of the MacB-family with demonstrable TolC-dependency.Citation
Lu W-J, Lin H-J, Janganan TK, Li C-Y, Chin W-C, Bavro VN, Lin H-T V (2018) 'ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function.', International Journal of Molecular Sciences, 19 (4), pp.-.Publisher
MDPIPubMed ID
29584668Additional Links
http://www.mdpi.com/1422-0067/19/4/1000Type
ArticleLanguage
enISSN
1661-6596ae974a485f413a2113503eed53cd6c53
10.3390/ijms19041000
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