Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.
AuthorsJanganan, Thamarai K.
Tzokov, Svetomir B.
Fagan, Robert P.
Hobbs, Jamie K.
Bullough, Per A.
MetadataShow full item record
AbstractClostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75-100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores.
CitationJanganan TK, Mullin N, Tzokov SB, Stringer S, Fagan RP, Hobbs JK, Moir A, Bullough PA (2016) 'Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.', Food Microbiology, 59 (), pp.205-12.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Green - can archive pre-print and post-print or publisher's version/PDF
- Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores.
- Authors: Díaz-González F, Milano M, Olguin-Araneda V, Pizarro-Cerda J, Castro-Córdova P, Tzeng SC, Maier CS, Sarker MR, Paredes-Sabja D
- Issue date: 2015 Jun 18
- Clostridium difficile exosporium cysteine-rich proteins are essential for the morphogenesis of the exosporium layer, spore resistance, and affect C. difficile pathogenesis.
- Authors: Calderón-Romero P, Castro-Córdova P, Reyes-Ramírez R, Milano-Céspedes M, Guerrero-Araya E, Pizarro-Guajardo M, Olguín-Araneda V, Gil F, Paredes-Sabja D
- Issue date: 2018 Aug
- Architecture and Self-Assembly of Clostridium sporogenes and Clostridium botulinum Spore Surfaces Illustrate a General Protective Strategy across Spore Formers.
- Authors: Janganan TK, Mullin N, Dafis-Sagarmendi A, Brunt J, Tzokov SB, Stringer S, Moir A, Chaudhuri RR, Fagan RP, Hobbs JK, Bullough PA
- Issue date: 2020 Jul 1
- The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly.
- Authors: Barra-Carrasco J, Olguín-Araneda V, Plaza-Garrido A, Miranda-Cárdenas C, Cofré-Araneda G, Pizarro-Guajardo M, Sarker MR, Paredes-Sabja D
- Issue date: 2013 Sep
- The ExsY protein is required for complete formation of the exosporium of Bacillus anthracis.
- Authors: Boydston JA, Yue L, Kearney JF, Turnbough CL Jr
- Issue date: 2006 Nov