Novel classes of fatty acid and retinol binding protein from nematodes
AffiliationUniversity of Glasgow
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AbstractParasitic nematodes have recently been found to produce proteins which represent two new classes of fatty acid and retinoid binding protein. The first is the nematode polyprotein allergens/antigens (NPAs) which, as their name suggests, are synthesised as large polyproteins which are subsequently cleaved at regularly spaced sites to form multiple copies of a fatty acid binding protein of approximately 14.5 kDa. Binding studies using molecular environment-sensitive fluorescent ligands have shown that the binding site is highly unusual, producing blue-shifting in fluorescence to an unprecedented degree, suggesting a remarkably non-polar environment and isolation from solvent water. Computer-based structural predictions and biophysical observations have identified the NPAs as highly helical proteins which might form a four helix bundle, so constitute a new class of lipid binding protein from animals. The second class, like the NPAs, binds both fatty acids and retinol, but with a higher affinity for the latter. These are also highly helical but are structurally distinct from the NPAs. The biological function of these new classes of protein are discussed in the context of both the metabolic requirements of the parasites and the possible role of the proteins in control of the immune and inflammatory environment of the tissue sites parasitised.
CitationMcDermott L, Cooper A, Kennedy MW (1999) 'Novel classes of fatty acid and retinol binding protein from nematodes', Molecular and Cellular Biochemistry, 192 (1-2), pp.67-75.
- The polyprotein lipid binding proteins of nematodes.
- Authors: Kennedy MW
- Issue date: 2000 Feb 9
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- Authors: Meenan NA, Ball G, Bromek K, Uhrín D, Cooper A, Kennedy MW, Smith BO
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- How helminth lipid-binding proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of schistosomes.
- Authors: McDermott L, Kennedy MW, McManus DP, Bradley JE, Cooper A, Storch J
- Issue date: 2002 May 28
- The ABA-1 allergen of the parasitic nematode Ascaris suum: fatty acid and retinoid binding function and structural characterization.
- Authors: Kennedy MW, Brass A, McCruden AB, Price NC, Kelly SM, Cooper A
- Issue date: 1995 May 23